In vivo chronic toxicology analysis. a, e Shows the unaltered brain pathology of control and GAE treated Zebrafish. b, f Shows the pathology of heart muscles in control and GAE treated Zebrafish. c, g Shows the unaltered muscle pathology of control and GAE treated Zebrafish. d, h Shows the pathology of liver cells in control and GAE treated Zebrafish. The pathology results confirm that GAE is not toxic to these major organs under chronic conditions. C-control, T-treated

Toxicology analysis in Tissue-Chip. The pathology studies of vital organs namely brain, heart, liver and muscle were analyzed in both control (a, c, e, g) and in treated (b, d, f, h) tissue chip. The treatment with algal extract 500 μg did not affect the tissue architecture. The results of tissue chip analysis also support GAE to be safe without toxicity. These tissues were developed and analysed for toxicity in Tissue-Chip Pentagrit, Chennai. C-control, T-treated

In vivo analysis of antitumor activity. a, b, c Histopathological analysis of muscle and tumor of tumor induced zebrafish. d, e, f Histopathological analysis of muscle and tumors from GAE treated tumor induced zebrafish. C-control, T-treated