- Title
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Cardiac Glycoside Glucoevatromonoside Induces Cancer Type-Specific Cell Death
- Authors
- Schneider, N.F.Z., Cerella, C., Lee, J.Y., Mazumder, A., Kim, K.R., de Carvalho, A., Munkert, J., Pádua, R.M., Kreis, W., Kim, K.W., Christov, C., Dicato, M., Kim, H.J., Han, B.W., Braga, F.C., Simões, C.M.O., Diederich, M.
- Source
- Full text @ Front Pharmacol
Glucoevatromonoside abrogates tumor formation in 3D culture and zebrafish xenografts. (A) Representative bright field images and 3D plots of spheroids generated after dose-dependent treatment of A549 by GEV (mean of three independent 4-day experiments ± SD). Quantification of tumor area and volume are shown; (B) A549 colony formation assays in the presence of increasing concentrations of GEV (10 days). Number of colonies, total, and average area are reported (mean of four independent experiments ± SD); (C) effect of GEV on tumor formation in a zebrafish A549 xenograft assay. CM-dil-stained A549 cells were treated for 30 h with 0, 10, 50, and 100 nM GEV and injected in the zebrafish yolk sac at 48 hpf. Representative images from at least 10 fish per condition. PBS injection served as a negative control. Fluorescent tumor mass was quantified and reported in the graph. Statistical analysis by ANOVA-one way; Dunnett's post-hoc test. Significance is reported as: *P < 0.05, ***P < 0.001, ****P < 0.0001 compared to controls. PHENOTYPE:
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