Transient GFP expression of linearised fads2 promoter (−1735/+19 bp) localised in yolk syncytial later (YSL) of zebrafish embryos. Lateral view of 26, 48, 72 and 96 hpf of transient transgene embryo injected with linearised fads2 promoter (−1735/+19 bp) showing increased expression in YSL (A,C,E,G) and their corresponding control (B,D,F,H). Cross-sectional view of a 120hpf control embryo (KN) and a 120hpf transient transgenic embryo (I,J) injected with linearised fads2 promoter (−1735/+19 bp) showing expression at YSL.

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