- Title
-
An In Toto Approach to Dissecting Cellular Interactions in Complex Tissues
- Authors
- Shah, P.K., Santella, A., Jacobo, A., Siletti, K., Hudspeth, A.J., Bao, Z.
- Source
- Full text @ Dev. Cell
Automated Ablation of Hair Cells in Zebrafish Neuromasts (A) Max projection of a single neuromast in a 3 dpf D. rerio larva expressing cldnb::H2B::mCherry (magenta) and myo6b::β-actin::GFP (green). Inset shows expanded view of the GFP-labeled hair cell stereocilia. wt, wild-type. Scale bar represents 10 μm. (B) Time series of polarity establishment in wild-type hair cell visualized by β-actin::GFP. Red dot indicates no evident polarity, red arrow indicates polarity. Scale bar represents 0.5 μm. (C) Distribution of hair cell polarity in wild-type neuromasts (N = 26). (D) Example sequence from the automated ablation of a hair cell (white arrow) and the subsequent maturation of the surviving sister (dashed ovals). Scale bar represents 10 μm. (E) Distribution of hair cell polarity in mock-ablated neuromasts, where a neighboring mature hair cell was ablated instead of one of the two newly born hair cells (N = 24). (F) Distribution of polarity in hair cells whose siblings were ablated soon after birth (N = 9). (G) Time series of early polarization in a hair cell whose sibling was ablated soon after birth. Annotations as in (B). Scale bar represents 0.5 μm. |
Reprinted from Developmental Cell, 43, Shah, P.K., Santella, A., Jacobo, A., Siletti, K., Hudspeth, A.J., Bao, Z., An In Toto Approach to Dissecting Cellular Interactions in Complex Tissues, 530-540.e4, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell