FIGURE SUMMARY
Title

Macrophages as drivers of an opportunistic infection

Authors
Vergunst, A.C., Carranza, N.L., Zhang, L., Gomes, M.C., Tasrini, Y., Meijer, A.H., O'Callaghan, D.
Source
Full text @ Microb Cell
Figure 1

FIGURE 1: <italic>B. cenocepacia</italic> requires macrophages for efficient replication and the induction of acute pro-inflammatory disease.

Zebrafish larvae are highly sensitive to infection with B. cenocepacia K56-2. Injection of a small inoculum (5 - 50 colony forming units) causes rapid pro-inflammatory fatal disease that requires the presence of macrophages, but not neutrophils. Macrophages are important for the observed increase in il1b expression. In the absence of macrophages, either using a Pu.1 morpholino knockdown approach or chemical ablation based on the Metronidazole / Nitroreductase system, B. cenocepacia K56-2 is unable to proliferate as in wildtype. This phenomenon was observed when the bacteria were injected intravenously and subcutaneously.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Microb Cell
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