- Title
-
Ultraconserved Sequences Associated with HoxD Cluster Have Strong Repression Activity
- Authors
- Kushawah, G., Mishra, R.K.
- Source
- Full text @ Genome Biol. Evol.
Reporter assay in zebrafish: Individual zebrafish embryos injected with different mouse CRs reporter constructs in equimolar concentration and imaged on dpf. GFP expression in the same embryo is shown at 2, 4, 6, and 8 dpf in the respective row. CR2 embryo shows high level of GFP expression during early embryogenesis and after 4 dpf GFP expression reduces drastically as compared with control embryo. CR1, CR3, and CR6 show comparable GFP expression as in the case of control even after 8 dpf. |
Reporter assay in zebrafish : Embryos of different stages injected with zebrafish CR constructs are imaged (phase contrast and GFP). Same embryo is followed for for up to 8 dpf in each row. CR2 shows a high level of GFP at 2dpf which reduces drastically after 4dpf. CR1,3 & 6 show comparable GFP levels as in the case of control even after 8dpf. |