FIGURE SUMMARY
- Title
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Identification of novel antigen candidates for a tuberculosis vaccine in the adult zebrafish (Danio rerio)
- Authors
- Myllymäki, H., Niskanen, M., Oksanen, K.E., Sherwood, E., Ahava, M., Parikka, M., Rämet, M.
- Source
- Full text @ PLoS One
In situ GFP expression in immunized zebrafish. AB fish were immunized with 12 μg of experimental or control vaccine plasmids, followed by electroporation. Seven days post-injection, the successful vaccinations and expression of the antigen-GFP fusion proteins were verified by fluorescence microscopy. The fluorescence resulting from the expression of the antigen-GFP fusion protein is seen in the dorsal muscle near the injection site. For each antigen, a representative example is shown. Non-immunized AB fish were used as a negative control. |
Acknowledgments
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Full text @ PLoS One