FIGURE SUMMARY
Title

IFT81 as a Candidate Gene for Nonsyndromic Retinal Degeneration

Authors
Dharmat, R., Liu, W., Ge, Z., Sun, Z., Yang, L., Li, Y., Wang, K., Thomas, K., Sui, R., Chen, R.
Source
Full text @ Invest. Ophthalmol. Vis. Sci.

IFT81 mutant in ift81hi409tg/hi409tg background displays ciliary defects: functional assay of the human IFT81 mutation in zebrafish ift81hi409tg/+ in-crosses. (A) Zebrafish embryos were injected with 50 pg human IFT81-C-Myc-flag or human c.1841T>C-IFT81 mutant-C-Myc-flag mRNA, Western blotting was performed using an anti–C-Myc antibody, β-actin was used as a control (day 0:6 hours after injection; day 1: 28 hours after injection). (B) Bright field images of day 3.5 wild-type like embryo (WT), partial rescued embryo (without kidney cyst but with post-vent increased curvature), and mutant embryo (with postvent increased curvature and kidney cyst; see the arrows and detailed inset in the box). (C) Phenotype distribution of zebrafish embryos injected with 50 pg IFT81 mRNA (WT), IFT81 mutant mRNA (MUT), and noninjected control displaying a significant (***P = 0.0006) reduction in the rescue potential of mutant IFT81 in comparison to WT.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.