Inhibitory activities of PCA against V. anguillarum C312 infection to zebrafish embryos. (A) PCA inhibits V. anguillarum C312 infection to zebrafish embryos. Zebrafish embryos were infected with V. anguillarum C312 in the absence or presence of different concentrations of PCA and the relative hatching percent of zebrafish embryos was calculated after 48 h infection. Error bars indicate the standard deviations of three measurements. ^∗P < 0.05. The error bars (SD) from the mean for three replicates are shown. (B) The typical hatching pictures of zebrafish embryos infected with V. anguillarum C312 in the absence or presence of different concentrations of PCA. All the pictures were taken with stereo microscope (ZEISS Stemi 2000C, German).