Rab5 plays a crucial role in apical extrusion of v-Src–expressing cells in the EVL of zebrafish embryos. (A) Confocal images of a Rab5-YFP–expressing zebrafish embryo (at 10–11 h postfertilization, 3 h after tamoxifen treatment) with RFP–v-Src in a mosaic manner. (Scale bar, 20 μm.) (B and C) Effect of expression of Rab5DN on apical extrusion of v-Src–expressing cells. Embryos were injected with the GFP–v-Src–expressing vector with or without the Rab5DN cassette. Representative images are shown in B. Arrows indicate apically extruded v-Src–expressing cells. (Scale bar, 10 μm.) (C) Quantification of apical extrusion of v-Src–expressing cells. Data are mean ± SD from four independent experiments. *P < 0.05; n = 490 and 364 cells.