- Title
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The novel protective role of P27 in MLN4924-treated gastric cancer cells
- Authors
- Zhang, Q., Hou, D., Luo, Z., Chen, P., Lv, B., Wu, L., Ma, Y., Chu, Y., Liu, H., Liu, F., Yu, S., Zhang, J., Yang, D., Liu, J.
- Source
- Full text @ Cell Death Dis.
P27 silencing enhanced the cytotoxicity of MLN4924 in gastric cancer cells. (a) The designed siRNA downregulated P27 in gastric cancer cells. AGS and MGC80-3 cells were transfected with NC or siP27 for 72 h and then subjected to immunoblotting analysis for expression of P27 and tubulin. (b and c) SiP27 enhanced the cytotoxicity of MLN4924 in gastric cancer cells. AGS and MGC80-3 cells transfected with NC or siP27 were treated with DMSO or 0.3 μM MLN4924. Cells were then subjected to stained by Live Dye (green) and propidium iodide (red) in vivo (bottom panel), or the CCK8 colorimetric assay (top panel). The dead cells (b) were indicated by red. (d) Smaller tumor size after combination treatment of the zebrafish xenograft models for AGS-GFP. AGS cells with GFP fluorescence transfected with NC or siP27 were transplanted into zebrafish embryos. Tumor sizes were indicated by the lateral fluorescent area. For panel b, n=4; for panel d, n=10. NC=Non-sense control siRNA; D=DMSO; M=MLN4924; si=siRNA for P27 |