FIGURE SUMMARY
- Title
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Mutational activation of BRAF confers sensitivity to transforming growth factor beta inhibitors in human cancer cells
- Authors
- Spender, L.C., Ferguson, G.J., Liu, S., Cui, C., Girotti, M.R., Sibbet, G., Higgs, E.B., Shuttleworth, M.K., Hamilton, T., Lorigan, P., Weller, M., Vincent, D.F., Sansom, O.J., Frame, M., Ten Dijke, P., Marais, R., Inman, G.J.
- Source
- Full text @ Oncotarget
TGFBR1 is required for tumour cell metastasis in xenografted zebrafish. a., b. A375(M2) cells stably transfected with a non-silencing shRNA control plasmid or two independent shRNA vectors targeting TGFBR1 were assessed by western blotting for a reduction in TGFBR1 expression and signalling in response to exogenous TGFβ addition a. and colony formation b.. c. Cells described in a. were labelled with mCherry and implanted into the Duct of Cuvier of zebrafish at 2 days post-fertilization (dpf). SB-431542 (1µM) was added to the egg water of the non-silencing (NS) + SB-431542 group. Confocal images were taken at 4 days post implantation (dpi). Arrows indicate invasive tumour cells, scale bar: 100 µm. d. Invasive cell numbers in tail fin of each zebrafish in each group. Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by the Tukey’s method for multiple comparison *** p < 0.001. Data are combined from four independent experiments and the total number of embryos (n) in each group is indicated. |
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Full text @ Oncotarget