Large-scale structural gonadotrope networks in the zebrafish pituitary.

Imaging of transgenic zebrafish pituitaries reveals that LH (a, LH:tagRFP line) and FSH (b,c, FSH:EGFP line) gonadotropes are arranged in three-dimensional networks. LH cells form a continuous population throughout the PPD (a). FSH cells are more loosely distributed throughout the PPD (b). A closer observation reveals that FSH cells form small aggregations in which cells contact each other (c). Both fish are adult females. Dorsal view, anterior-up, white line marks pituitary borders, PI - pars intermedia, RPD - rostral pars distalis. Bars in (a,b) -100 µm, (c) -20 µm.