FIGURE SUMMARY
- Title
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Ruthenium-Caged Antisense Morpholinos for Regulating Gene Expression in Zebrafish Embryos
- Authors
- Griepenburg, J.C., Rapp, T.L., Carroll, P.J., Eberwine, J., Dmochowski, I.J.
- Source
- Full text @ Chem Sci
Representative confocal micrographs of 24–28 hpf zebrafish embryos, showing chd knockdown phenotypes depending on experimental protocol. (A) Wildtype embryo, uninjected. (B) Ru-MO-chd, incubated in the dark, showing normal development. (C) Ru-MO-chd, irradiated for 5 min at 1 hpf with 450 nm light, showing chd knockdown phenotype. (D) Bis-azido MO-chd showing chd knockdown phenotype. All embryos were injected at 1-cell stage and imaged with a 10× air objective (Olympus UPlanSApo, NA = 0.40). (E) Percentage of embryos showing wildtype or degree of phenotypic response. PHENOTYPE:
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Full text @ Chem Sci