Screening data obtained using the 3D printed orientation tools. Shown are illustrative examples of embryos within agarose cavities generated with 3D printed orientation tools. All images shown derive from single 96 well plates with laterally or dorsally oriented embryos, respectively (see also Additional file 3). (A, B) Cropped extended focus bright field images of 48 hpf zebrafish embryos: (A) lateral and (B) dorsal views. (C) Cropped maximum projections of deconvolved z-stacks of kidney regions of 48 hpf embryos of the Tg(wt1b:EGFP) transgenic line.

Examples of data from entire 96 well plates containing oriented embryos. Shown are laterally and dorsally oriented embryos from two single 96 well plates. (A-D) lateral orientation and (E-H) dorsal orientation. To illustrate average orientation accuracy within a single plate originating from a routine experiment, embryos were divided in three categories: (A, E) well oriented (lateral: 92.7%, dorsal: 78.1%), (B, F) slightly tilted (lateral: 6.3%; dorsal: 16.7%) and (C, G) failed orientation (lateral: 1.0%; dorsal: 5.2%). Embryos damaged during pipetting or orienting were included in the failed category. Thumbnail images in D and H show overviews of entire 96 well plates.