FIGURE SUMMARY
Title

Normalization of RNA-sequencing data from samples with varying mRNA levels

Authors
Aanes, H., Winata, C., Moen, L.F., Østrup, O., Mathavan, S., Collas, P., Rognes, T., and Aleström, P.
Source
Full text @ PLoS One
Fig. 1

Experimental design. Total RNA from 5 developmental stages pre- and post-ZGA was isolated and kanamycin polyA+ RNA was used to adjust for differences in RNA yield. PolyA+ RNA was isolated and four cDNA libraries were generated to compare qPCR results using different template and primers.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.