FIGURE SUMMARY
Title

Lampreys have a single gene cluster for the fast skeletal Myosin heavy chain gene family

Authors
Ikeda, D., Ono, Y., Hirano, S., Kan-No, N., and Watabe, S.
Source
Full text @ PLoS One
Fig. 3

Transient expression of the Japanese lamprey MYH1-3kb-hrGFP and MYH2-3kb-DsRed transgenes in zebrafish embryos.

A, B: Lateral view showing the expression of the MYH1-3kb-hrGFP in the trunk myotome of zebrafish embryos at 1 (A) and 3 (B) days post-fertilization (dpf). C: Lateral view showing the expression of the MYH2-3kb-DsRed in the trunk myotome of an embryo at 3 dpf. D: Lateral view showing the expression of the MYH1-3kb-hrGFP and MYH2-3kb-DsRed in the trunk myotome of an embryo at 3 dpf. Two muscle fibers clearly co-expressed the 2 transgenes in the same cells (merged, arrowheads). E-G: Lateral (E) and right (F) and left (G) ventrolateral side views showing the expression of the MYH1-3kb-hrGFP in cranial muscles of an embryo at 5 dpf. H-K: Transverse sections at the middle trunk of an embryo at 5 dpf. Cells were labeled with an antibody against GFP (H) and F59 antibody raised against chicken slow skeletal MYH (I). Differential interference contrast (DIC) microscopy image (J) and merged image from panels H, I and J (K). Asterisks indicate crosstalk signals due to strong GFP expression in the red channel. Note that superficial slow skeletal muscle fibers were labeled with F59, and the MYH1-3kb-hrGFP transgene was found to be expressed in fast muscle fibers. Scale bar = 20 μm.
Fig. S4

Transient expression of the Japanese lamprey MYH1-2kb-hrGFP, MYH1-5kb-hrGFP, MYH2-2kb-DsRed and MYH2-5kb-DsRed transgenes in zebrafish embryos. Lateral view showing the expression of the MYH1-2kb-hrGFP (A), MYH2-2kb-DsRed (B), MYH1-5kb-hrGFP (C) and MYH2-5kb-DsRed (D) in the trunk myotome of zebrafish embryos at 3 days post-fertilization. Arrowheads indicate autofluorescence due to pigment cells on the embryo surface.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
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