FIGURE SUMMARY
- Title
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Identification and Characterization of a Novel Hemolysis-Related Gene in Streptococcus suis Serotype 2
- Authors
- Zheng, J.X., Li, Y., Zhang, H., Fan, H.J., and Lu, C.P.
- Source
- Full text @ PLoS One
Construction and confirmation of the ZY05719Δhhly3 mutant strain. (A) Construction strategy of hhly3 gene knockout mutant by allelic replacement. (B) Localization analysis of Hhly3. Western blot analysis of membrane-associated proteins with anti-rHhly3 rabbit polyclonal antibodies. (C) PCR confirmation of knockout mutant strain Δhhly3. The specific primer pair IN1/IN2 was used to detect the presence of the hhly3 gene in the SS2 genome. The flanking primer pair OUT1/OUT2 located outside LA and RA was designed to confirm double-crossover recombination in the mutant by combined PCR detection. |
Acknowledgments
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Full text @ PLoS One