Consequences of 20Gy at 25 cGy/min vs 800cGy/min in non-transplanted fish.

A, Kaplan-Meier survival curves of adult zebrafish irradiated with 20Gy at either 25cGy/min (LDR) or 800cGy/min (HDR). Pooled data from two independent trials, each trial composed of ten fish per group. p = .356 (Log Rank Test). B, TUNEL staining (above), and H&E staining (below) of adult zebrafish kidneys harvested one day after 20Gy radiation delivered at either 25cGy/min or 800cGy/min.

Anatomical characteristics of hematopoietic reconstitution 9dpt.

A) Fish were irradiated with 20 Gy at either a high dose rate of 800 cGy/min (HDR) or lower dose rate of 25 cGy/min (LDR), and transplanted with Bactin2:EGFP WKM cells. 9 days after transplant (dpt), live fish were photographed to record whole-body GFP signal. Mean gray values were obtained from manual photo composites of each fish. Representative photographs show the approximate mean gray value of each group. Data shows results pooled from two independent biological experiments, for a composite total of 15–16 fish per group. B) Fresh kidneys were photographed to detect GFP signal 9 dpt, six fish per group. Sites with exceptionally high concentrations of donor-derived cells are indicated with yellow arrowheads. C) Tg(sdf-1a:DsRed) recipients were transplanted with bactin2:EGFP WKM cells. 9 dpt, kidneys from each group were photographed. Manual photo composites document sdf-1a:DsRed and bactin2:EGFP expression. Region of enlargement was chosen based on anatomical similarity to indicated regions in panel B. No HDR-specific expression patterns of sdf-1a were noted. Kidneys of three recipients per group were photographed and analyzed. Scale, 200 μM.