- Title
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A small molecule inhibitor of redox-regulated protein translocation into mitochondria
- Authors
- Dabir, D.V., Hasson, S.A., Setoguchi, K., Johnson, M.E., Wongkongkathep, P., Douglas, C.J., Zimmerman, J., Damoiseaux, R., Teitell, M.A., and Koehler, C.M.
- Source
- Full text @ Dev. Cell
Embryos (3 hpf) were treated with 2.5 μM MitoBloCK-6 (B, E, and H) or 1% DMSO (A, D, and G) or embryos were injected with an ATG morpholino against ALR (C and F). Development was visualized by microscopy at 72 hpf (A–C). Erythrocytes were visualized by o-dianisidine staining at 72 hpf (D–F); arrows indicate regions of red blood cell accumulation in wild-type fish. Fluorescence microscopy of zebrafish hearts (72 hpf) that contained a mitochondrial-targeted DsRed included embryos treated with 1% DMSO (G), 2.5 μM MitoBloCK-6 (H), and buffer only (I). EXPRESSION / LABELING:
PHENOTYPE:
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A combination of MitoBloCK-6 and the ATG morpholino against Erv1 at lower concentrations are additive in impairing zebrafish development. Embryos were treated with combinations of MitoBloCK-6 and the ATG morpholino against Erv1 at the indicated concentrations. Development was visualized by microscopy at 72 hpf as described in Figure 7. |
Reprinted from Developmental Cell, 25(1), Dabir, D.V., Hasson, S.A., Setoguchi, K., Johnson, M.E., Wongkongkathep, P., Douglas, C.J., Zimmerman, J., Damoiseaux, R., Teitell, M.A., and Koehler, C.M., A small molecule inhibitor of redox-regulated protein translocation into mitochondria, 81-92, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell