- Title
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Stable transgenesis in the marine annelid Platynereis dumerilii sheds new light on photoreceptor evolution
- Authors
- Backfisch, B., Veedin Rajan, V.B., Fischer, R.M., Lohs, C., Arboleda, E., Tessmar-Raible, K., and Raible, F.
- Source
- Full text @ Proc. Natl. Acad. Sci. USA
Molecular specification of Platynereis noncephalic photoreceptors and presence of r-opsin orthologs in the zebrafish neuromasts. (A-I) Codetection of Platynereis r-opsin (red arrowheads) and indicated transcripts (purple arrowheads) by double in situ hybridization. Coexpression is indicated by black arrows. (A–C) Expression in the adult ventral trunk. (D–G) Expression in 8-d tail regenerates (trunk and parapodia). (H and I) Expression in adult parapodia. (J–L) Expression of opn4x-2 and opn4m-2 in the zebrafish neuromast. (J) Live image of neuromast cells (green arrowheads) in the brn3c::egfp strain 6 d after fertilization; (K and L) corresponding labeling of neuromast cells (purple arrowheads) with the opn4x-2 and opn4m-2 riboprobes. (A–G) Ventral views, anterior to the left; (H and I) frontal views, dorsal to the top. (J–L) Lateral views, anterior to the left. See text for details. |