Fig. 2

Expression of heparan sulfate (HS) 6-O-sulfotransferase (6-OST) genes during early zebrafish development. A-E: Expression of 6-OST-1a at two cell (A), 60% epiboly (B), 7 somite (C,D), and 18 somite (E) stages. F-J: Expression of 6-OST-1b at two cell (F), 60% epiboly (G), 7 somite (H,I), and 16 somite (J) stages. K-O: Expression of 6-OST-2 at two cell (K), 60% epiboly (L), 7 somite (M,N), and 18 somite (O) stages. P-T: Expression of 6-OST-3 at two cell (P), 60% epiboly (Q), 10 somite (R,S), and 18 somite (T) stages. DFC, dorsal forerunner cells; E, eye; HB, hindbrain; KV, Kupffer′s vesicle; S, somites; SC, spinal cord.

Fig. 3

Expression of heparan sulfate (HS) 6-O-sulfotransferase (6-OST) genes at 24 hours postfertilization (hpf) and 48 hpf. A,B: Expression of 6-OST-1a during 24 hpf (A) and 48 hpf (B). C,D: Expression of 6-OST-1b during 24 hpf (C) and 48 hpf (D). E,F: Expression of 6-OST-2 during 24 hpf (E) and 48 hpf (F). G,H: Expression of 6-OST-3 during 24 hpf (G) and 48 hpf (H). BA, branchial arches; E, eye; FB, forebrain; HB, hindbrain; MB, midbrain; OT, otic vesicle; PF, pectoral fin; S, somites; SC, spinal cord.

Fig. 4

Expression of 6-O-sulfotransferase (6-OST) family members in the 36 hours postfertilization (hpf) brain. A,B: Dorsal (A) and lateral (B) expression of 6-OST-1a. C,D: Dorsal (C) and lateral (D) expression of 6-OST-1b. E,F: Dorsal (E) and lateral (F) expression of 6-OST-2. G,H: Dorsal (G) and lateral (H) expression of 6-OST-3. CMZ, ciliary marginal zone; EL, eye lense; FB, forebrain; HB, hindbrain; MB, midbrain; OT, otic vesicle.

Fig. 5

Expression of 6-O-sulfotransferase (6-OST) family members in the developing eye. A-D: Expression of 6-OST-1a in eye at 18 somite (A), 24 hpf (B), and 48 hpf (C, lateral and D, dorsal) stages. E-H: Expression of 6-OST-1b at 18 somite (E), 24 hpf (F), and 48 hpf (G, lateral and H, dorsal) stage. I-L: Expression of 6-OST-2 in eye at 18 somite (I), 24 hpf (J), and 48 hpf (K, lateral and L, dorsal) stages. M-P: Expression of 6-OST-3 in eye at 18 somite (M), 24 hpf (N), and 48 hpf (O, lateral and P, dorsal) stages. EL, eye lens; NR, neural retina; RGC, retinal ganglion cells.

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