ZFIN Lab: Schaaf Lab

ZFIN ID: ZDB-LAB-170906-3

Schaaf Lab

PI/Director:	Schaaf, Marcel J. M.
Contact Person:	Schaaf, Marcel J. M.
Email:	m.j.m.schaaf@biology.leidenuniv.nl
URL:	http://www.universiteitleiden.nl/en/staffmembers/marcel-schaaf
Address:	Institute of Biology, Leiden University Gorlaeus Laboratory, room BS1.03 Einsteinweg 55, 2333 CC Leiden
Country:	Netherlands
Phone:	+31 71 5274975
Fax:
Line Designation:	ibl

GENOMIC FEATURES ORIGINATING FROM THIS LAB

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STATEMENT OF RESEARCH INTERESTS

We study the molecular mechanisms of glucocorticoid signaling. Glucocorticoids are steroid hormones that are secreted upon stress and have strong immune-suppressive actions. The main endogenous glucocorticoid hormone in humans and in fish is cortisol, and synthetic analogs of this hormone like prednisone and dexamethasone are widely used clinically as anti-inflammatory drugs. We have cloned and characterized the zebrafish glucocorticoid receptor (GR), and studied the role of a receptor splice variant GRbeta. Currently, our research focuses on the cell type-specific molecular mechanisms underlying the immune-suppressive actions of glucocorticoids.

LAB MEMBERS

Gora, Radek Graduate Student	Keizer, Veer Graduate Student	Luchtenburg, Floris Graduate Student
Nowik, Natalia Graduate Student	Sanchez, Munoz Graduate Student	Xie, Yufei Graduate Student

ZEBRAFISH PUBLICATIONS OF LAB MEMBERS

Faught, E., Schaaf, M.J.M. (2023) Molecular mechanisms of the stress-induced regulation of the inflammatory response in fish. General and comparative endocrinology. 345:114387

Faught, E., Schaaf, M.J.M. (2023) The mineralocorticoid receptor plays a crucial role in macrophage development and function. Endocrinology. 164(10):

Xiong, Y., Halima, M., Che, X., Zhang, Y., Schaaf, M.J.M., Li, M., Gao, M., Guo, L., Huang, Y., Cui, X., Wang, M. (2022) Steamed Panax notoginseng and its Saponins Inhibit the Migration and Induce the Apoptosis of Neutrophils in a Zebrafish Tail-Fin Amputation Model. Frontiers in pharmacology. 13:946900

Rock, S., Rodenburg, F., Schaaf, M.J.M., Tudorache, C. (2022) Detailed Analysis of Zebrafish Larval Behaviour in the Light Dark Challenge Assay Shows That Diel Hatching Time Determines Individual Variation. Frontiers in Physiology. 13:827282

Xie, Y., Papadopoulou, P., de Wit, B., d'Engelbronner, J.C., van Hage, P., Kros, A., Schaaf, M.J.M. (2022) Two Types of Liposomal Formulations Improve the Therapeutic Ratio of Prednisolone Phosphate in a Zebrafish Model for Inflammation. Cells. 11(4):

Gora, R.J., de Jong, B., van Hage, P., Rhiemus, M.A., van Steenis, F., van Noort, J., Schmidt, T., Schaaf, M.J.M. (2021) Analysis of the H-Ras mobility pattern in vivo shows cellular heterogeneity inside epidermal tissue. Disease models & mechanisms. 15(2):

Bernardello, M., Gora, R.J., Van Hage, P., Castro-Olvera, G., Gualda, E.J., Schaaf, M.J.M., Loza-Alvarez, P. (2021) Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy. Biomedical Optics Express. 12:6205-6227

Davis, R., Luchtenburg, F., Richardson, M., Schaaf, M., Tudorache, C., Slabbekoorn, H. (2021) The importance of individual variation for the interpretation of behavioural studies: ethanol effects vary with basal activity level in zebrafish larvae. Psychopharmacology. 238(11):3155-3166

Nowik, N., Prajsnar, T.K., Przyborowska, A., Rakus, K., Sienkiewicz, W., Spaink, H.P., Podlasz, P. (2021) The Role of Galanin during Bacterial Infection in Larval Zebrafish. Cells. 10(8):

Xie, Y., Xie, J., Meijer, A.H., Schaaf, M.J.M. (2021) Glucocorticoid-Induced Exacerbation of Mycobacterial Infection Is Associated With a Reduced Phagocytic Capacity of Macrophages. Frontiers in immunology. 12:618569

Sommer, F., Torraca, V., Xie, Y., In 't Veld, A.E., Willemse, J., Meijer, A.H. (2021) Disruption of Cxcr3 chemotactic signaling alters lysosomal function and renders macrophages more microbicidal. Cell Reports. 35:109000

He, J., Ding, Y., Nowik, N., Jager, C., H Eeza, M.N., Alia, A., Baelde, H.J., Spaink, H.P. (2021) Leptin deficiency affects glucose homeostasis and results in adiposity in zebrafish. The Journal of endocrinology. 249(2):125-134

Hosseini, R., Lamers, G.E.M., Bos, E., Hogendoorn, P.C.W., Koster, A.J., Meijer, A.H., Spaink, H.P., Schaaf, M.J.M. (2021) The adapter protein Myd88 plays an important role in limiting mycobacterial growth in a zebrafish model for tuberculosis. Virchows Archiv : an international journal of pathology. 479(2):265-275

Xie, Y., Meijer, A.H., Schaaf, M.J.M. (2021) Modeling Inflammation in Zebrafish for the Development of Anti-inflammatory Drugs. Frontiers in cell and developmental biology. 8:620984

Jaikumar, G., Slabbekoorn, H., Sireeni, J., Schaaf, M., Tudorache, C. (2020) The role of the Glucocorticoid Receptor in the Regulation of Diel Rhythmicity. Physiology & behavior. 223:112991

He, M., Halima, M., Xie, Y., Schaaf, M.J.M., Meijer, A.H., Wang, M. (2020) Ginsenoside Rg1 Acts as a Selective Glucocorticoid Receptor Agonist with Anti-Inflammatory Action without Affecting Tissue Regeneration in Zebrafish Larvae. Cells. 9(5):

van den Bos, R., Cromwijk, S., Tschigg, K., Althuizen, J., Zethof, J., Whelan, R., Flik, G., Schaaf, M. (2020) Early Life Glucocorticoid Exposure Modulates Immune Function in Zebrafish (Danio rerio) Larvae. Frontiers in immunology. 11:727

Sireeni, J., Bakker, N., Jaikumar, G., Obdam, D., Slabbekoorn, H., Tudorache, C., Schaaf, M. (2020) Profound effects of glucocorticoid resistance on anxiety-related behavior in zebrafish adults but not in larvae. General and comparative endocrinology. 292:113461

Puyskens, A., Stinn, A., van der Vaart, M., Kreuchwig, A., Protze, J., Pei, G., Klemm, M., Guhlich-Bornhof, U., Hurwitz, R., Krishnamoorthy, G., Schaaf, M., Krause, G., Meijer, A.H., Kaufmann, S.H.E., Moura-Alves, P. (2019) Aryl Hydrocarbon Receptor Modulation by Tuberculosis Drugs Impairs Host Defense and Treatment Outcomes. Cell Host & Microbe. 27(2):238-248.e7

Brun, N.R., van Hage, P., Hunting, E.R., Haramis, A.G., Vink, S.C., Vijver, M.G., Schaaf, M.J.M., Tudorache, C. (2019) Polystyrene nanoplastics disrupt glucose metabolism and cortisol levels with a possible link to behavioural changes in larval zebrafish. Communications biology. 2:382

Xie, Y., Tolmeijer, S., Oskam, J.M., Tonkens, T., Meijer, A.H., Schaaf, M.J.M. (2019) Glucocorticoids inhibit macrophage differentiation towards a pro-inflammatory phenotype upon wounding without affecting their migration. Disease models & mechanisms. 12(5):

Luchtenburg, F.J., Schaaf, M.J.M., Richardson, M.K. (2019) Functional characterization of the cannabinoid receptors 1 and 2 in zebrafish larvae using behavioral analysis. Psychopharmacology. 236(7):2049-2058

Palstra, A.P., Mendez, S., Dirks, R.P., Schaaf, M.J.M. (2019) Cortisol Acting Through the Glucocorticoid Receptor Is Not Involved in Exercise-Enhanced Growth, But Does Affect the White Skeletal Muscle Transcriptome in Zebrafish (Danio rerio). Frontiers in Physiology. 9:1889

Tudorache, C., Slabbekoorn, H., Robbers, Y., Hin, E., Meijer, J.H., Spaink, H.P., Schaaf, M.J.M. (2018) Biological clock function is linked to proactive and reactive personality types. BMC Biology. 16:148

He, M., Huang, X., Liu, S., Guo, C., Xie, Y., Meijer, A.H., Wang, M. (2018) The Difference between White and Red Ginseng: Variations in Ginsenosides and Immunomodulation. Planta Medica. 84(12-13):845-854

Schaaf, M.J.M. (2017) Nuclear receptor research in zebrafish. Journal of molecular endocrinology. 59:R65-R76

Chatzopoulou, A., Schoonheim, P.J., Torraca, V., Meijer, A.H., Spaink, H.P., Schaaf, M.J. (2017) Functional analysis reveals no transcriptional role for the glucocorticoid receptor β-isoform in zebrafish. Molecular and Cellular Endocrinology. 447:61-70

Hosseini, R., Lamers, G.E., Soltani, H.M., Meijer, A.H., Spaink, H.P., Schaaf, M.J. (2016) Efferocytosis and extrusion of leukocytes determine the progression of early mycobacterial pathogenesis. Journal of Cell Science. 129(18):3385-95

Chatzopoulou, A., Heijmans, J.P., Burgerhout, E., Oskam, N., Spaink, H.P., Meijer, A.H., Schaaf, M.J. (2016) Glucocorticoid-induced attenuation of the inflammatory response in zebrafish. Endocrinology. 157(7):2772-84

Chatzopoulou, A., Roy, U., Meijer, A.H., Alia, A., Spaink, H.P., Schaaf, M.J. (2015) Transcriptional and Metabolic Effects of Glucocorticoid Receptor α and β Signaling in Zebrafish. Endocrinology. 156(5):1757-69

Neo, Y.Y., Parie, L., Bakker, F., Snelderwaard, P., Tudorache, C., Schaaf, M., Slabbekoorn, H. (2015) Behavioral changes in response to sound exposure and no spatial avoidance of noisy conditions in captive zebrafish. Frontiers in behavioral neuroscience. 9:28

Tudorache, C., Ter Braake, A., Tromp, M., Slabbekoorn, H., Schaaf, M.J. (2015) Behavioural and physiological indicators of stress coping styles in larval zebrafish. Stress (Amsterdam, Netherlands). 18(1):121-8

Hosseini, R., Lamers, G.E., Hodzic, Z., Meijer, A.H., Schaaf, M.J., Spaink, H.P. (2014) Correlative light and electron microscopy imaging of autophagy in a zebrafish infection model. Autophagy. 10(10):1844-57

Tudorache, C., Schaaf, M.J., and Slabbekoorn, H. (2013) Covariation between behaviour and physiology indicators of coping style in zebrafish (Danio rerio). The Journal of endocrinology. 219(3):251-258

Ziv, L., Muto, A., Schoonheim, P.J., Meijsing, S.H., Strasser, D., Ingraham, H.A., Schaaf, M.J., Yamamoto, K.R., and Baier, H. (2013) An affective disorder in zebrafish with mutation of the glucocorticoid receptor. Molecular psychiatry. 18(6):681-91

Kanwal, Z., Zakrzewska, A., den Hertog, J., Spaink, H.P., Schaaf, M.J., and Meijer, A.H. (2013) Deficiency in Hematopoietic Phosphatase Ptpn6/Shp1 Hyperactivates the Innate Immune System and Impairs Control of Bacterial Infections in Zebrafish Embryos. Journal of immunology (Baltimore, Md. : 1950). 190(4):1631-1645

Schoonheim, P.J., Chatzopoulou, A., and Schaaf, M.J. (2010) The zebrafish as an in vivo model system for glucocorticoid resistance. Steroids. 75(12):918-925

Schaaf, M.J., Koopmans, W.J., Meckel, T., van Noort, J., Snaar-Jagalska, B.E., Schmidt, T.S., and Spaink, H.P. (2009) Single-molecule microscopy reveals membrane microdomain organization of cells in a living vertebrate. Biophysical journal. 97(4):1206-1214

van der Vaart, M., and Schaaf, M.J. (2009) Naturally occurring C-terminal splice variants of nuclear receptors. Nuclear receptor signaling. 7:e007

Schaaf, M.J., Chatzopoulou, A., and Spaink, H.P. (2009) The zebrafish as a model system for glucocorticoid receptor research. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 153(1):75-82

Brittijn, S.A., Duivesteijn, S.J., Belmamoune, M., Bertens, L.F., Bitter, W., Debruijn, J.D., Champagne, D.L., Cuppen, E., Flik, G., Vandenbroucke-Grauls, C.M., Janssen, R.A., de Jong, I.M., de Kloet, E.R., Kros, A., Meijer, A.H., Metz, J.R., van der Sar, A.M., Schaaf, M.J., Schulte-Merker, S., Spaink, H.P., Tak, P.P., Verbeek, F.J., Vervoordeldonk, M.J., Vonk, F.J., Witte, F., Yuan, H., and Richardson, M.K. (2009) Zebrafish development and regeneration: new tools for biomedical research. The International journal of developmental biology. 53(5-6):835-850

Schaaf, M.J., Champagne, D., van Laanen, I.H., van Wijk, D.C., Meijer, A.H., Meijer, O.C., Spaink, H.P., and Richardson, M.K. (2008) Discovery of a functional glucocorticoid receptor beta-isoform in zebrafish. Endocrinology. 149(4):1591-1599