ZFIN ID: ZDB-LAB-160721-1
Jusuf Lab
PI/Director: Jusuf, Patricia
Contact Person: Jusuf, Patricia
Email: stefanie.dudczig@unimelb.edu.au
Address: School of Biosciences The University of Melbourne Biosciences 5/ Building 184 Parkville, Victoria 3010 Australia
Country: Australia
Phone: +61 3 8344 9519
Line Designation: uom

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Our group is interested in understanding the genetic networks that co-ordinate the generation of different types of neurons in the central nervous system of vertebrates. We use zebrafish as a model and focus on neural specification in the retina in the eye, which has a relatively simpler organisation compared to other CNS regions.

Zebrafish grow quickly and are optically transparent, allowing us to track individual cells in the forming embryo. By visualising gene expression with fluorescent reporter protein, we can study how timing and interactions of genes within developing progenitors influence what types of neurons they can generate. We can also assess the consequences of gene manipulations including those that mimic human disorders as well as study how and why the zebrafish are able to completely regenerate neurons and neural function after injury in adults.

Caceres Valez, Paolin Post-Doc Krylov, Aaron Graduate Student Lawler, Clancy Graduate Student
Veen, Kellie Graduate Student Wallace, Lachlan Graduate Student Xie, Jiaheng Graduate Student

Wood, A.J., Lin, C.H., Li, M., Nishtala, K., Alaei, S., Rossello, F., Sonntag, C., Hersey, L., Miles, L.B., Krisp, C., Dudczig, S., Fulcher, A.J., Gibertini, S., Conroy, P.J., Siegel, A., Mora, M., Jusuf, P., Packer, N.H., Currie, P.D. (2021) FKRP-dependent glycosylation of fibronectin regulates muscle pathology in muscular dystrophy. Nature communications. 12:2951
Brandli, A., Dudczig, S., Currie, P.D., Jusuf, P.R. (2021) Photoreceptor ablation following ATP induced injury triggers Müller glia driven regeneration in zebrafish. Experimental Eye Research. 207:108569
Xie, J., Jusuf, P.R., Bui, B.V., Dudczig, S., Sztal, T.E., Goodbourn, P.T. (2021) Altered Visual Function in a Larval Zebrafish Knockout of Neurodevelopmental Risk Gene pdzk1. Investigative ophthalmology & visual science. 62:29
Xie, J., Jusuf, P.R., Bui, B.V., Goodbourn, P.T. (2019) Experience-dependent development of visual sensitivity in larval zebrafish. Scientific Reports. 9:18931
Xie, J., Goodbourn, P.T., Bui, B.V., Sztal, T.E., Jusuf, P.R. (2019) Correspondence Between Behavioral, Physiological, and Anatomical Measurements of Visual Function in Inhibitory Neuron-Ablated Zebrafish. Investigative ophthalmology & visual science. 60:4681-4690
Ehrlich, O., Karamalakis, A., Krylov, A.J., Dudczig, S., Hassell, K.L., Jusuf, P.R. (2019) Clove Oil and AQUI-S Efficacy for Zebrafish Embryo, Larva, and Adult Anesthesia. Zebrafish. 16(5):451-459
Xie, J., Jusuf, P.R., Goodbourn, P.T., Bui, B.V. (2019) Electroretinogram Recording in Larval Zebrafish using A Novel Cone-Shaped Sponge-tip Electrode. Journal of visualized experiments : JoVE. (145):
Goldshmit, Y., Tang, J.K.K.Y., Siegel, A.L., Nguyen, P.D., Kaslin, J., Currie, P.D., Jusuf, P.R. (2018) Different Fgfs have distinct roles in regulating neurogenesis after spinal cord injury in zebrafish. Neural Development. 13:24
Dudczig, S., Currie, P.D., Jusuf, P.R. (2017) Developmental and adult characterization of secretagogin expressing amacrine cells in zebrafish retina. PLoS One. 12:e0185107
Ng Chi Kei, J., Currie, P.D., Jusuf, P.R. (2017) Fate bias during neural regeneration adjusts dynamically without recapitulating developmental fate progression. Neural Development. 12:12
Goodings, L., He, J., Wood, A., Harris, W.A., Currie, P.D., Jusuf, P.R. (2017) In vivo expression of Nurr1/Nr4a2a in developing retinal amacrine subtypes in zebrafish Tg(nr4a2a:eGFP) transgenics. The Journal of comparative neurology. 525(8):1962-1979
Dudczig, S., Currie, P.D., Poggi, L., Jusuf, P.R. (2017) In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences. Journal of visualized experiments : JoVE. (121)
Ng Chi Kei, J., Dudczig, S., Currie, P.D., Jusuf, P.R. (2016) Feedback from each retinal neuron population drives expression of subsequent fate determinant genes without influencing the cell cycle exit timing. The Journal of comparative neurology. 524(13):2553-66
Ng, J., Currie, P., Jusuf, P. R. (2014) The Regenerative Potential of the Vertebrate Retina: Lessons from the Zebrafish. Regenerative Biology of the Eye. :49-82
Jusuf, P., Harris, W.A., and Poggi, L. (2013) Preparation of transgenic zebrafish embryos for imaging the developing retina. Cold Spring Harbor protocols. 2013(3):pdb.prot073536
Jusuf, P., Harris, W.A., and Poggi, L. (2013) Imaging retinal progenitor lineages in developing zebrafish embryos. Cold Spring Harbor protocols. 2013(3):pdb.prot073544
Jusuf, P.R., Albadri, S., Paolini, A., Currie, P.D., Argenton, F., Higashijima, S., Harris, W.A., and Poggi, L. (2012) Biasing Amacrine Subtypes in the Atoh7 Lineage through Expression of Barhl2. The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(40):13929-13944
Goldshmit, Y., Sztal, T.E., Jusuf, P.R., Hall, T.E., Nguyen-Chi, M., and Currie, P.D. (2012) Fgf-dependent glial cell bridges facilitate spinal cord regeneration in zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(22):7477-7492
Jusuf, P.R., Almeida, A.D., Randlett, O., Joubin, K., Poggi, L., and Harris, W.A. (2011) Origin and determination of inhibitory cell lineages in the vertebrate retina. The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(7):2549-2562
Jusuf, P.R., and Harris, W.A. (2009) Ptf1a is expressed transiently in all types of amacrine cells in the embryonic zebrafish retina. Neural Development. 4:34
Vitorino, M., Jusuf, P.R., Maurus, D., Yukiko, Y., Higashijima, S.I., and Harris, W.A. (2009) Vsx2 in the zebrafish retina: restricted lineages through derepression. Neural Development. 4:14