ZFIN ID: ZDB-LAB-130828-1
Long noncoding RNAs in vertebrate development
PI/Director: Shkumatava, Alena
Contact Person: Shkumatava, Alena
Email: alena.shkumatava@curie.fr
URL: http://ugbdd.curie.fr/en/article/001066-long-intervening-noncoding-rnas-lincrnas-vertebrate-development
Address: Equipe Shkumatava Unité de Génétique et Biologie du Développement Institut Curie 26, rue d'Ulm 75005 Paris, France
Country: France
Phone: +33-156-246897
Fax:
Line Designation: None assigned


GENOMIC FEATURES ORIGINATING FROM THIS LAB No data available


STATEMENT OF RESEARCH INTERESTS
The human genome encodes a large number of long intervening noncoding RNAs (lincRNAs): What are they? Do they have any biological functions? Using a combination of comparative genomics, zebrafish genetics and molecular biology, we are investigating whether lincRNAs control normal embryonic development. Perturbations of two lincRNAs (cyrano and megamind) caused dramatic developmental defects in zebrafish embryos including brain and eye abnormalities. These defects could be rescued when the missing zebrafish version of a lincRNA was replaced by a human equivalent, indicating that these molecules have conserved functions. In the future, we will focus on finding more lincRNAs that have crucial roles in development. We will decipher how they function within the cell, and which cellular components they partner with to control development. Ultimately, we aim to understand how these mysterious molecules control human development.


LAB MEMBERS
Graindorge, Antoine Post-Doc Bitetti, Angelo Graduate Student Eckert, Hélène Technical Staff


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Fontana, B.D., Reichmann, F., Tilley, C.A., Lavlou, P., Shkumatava, A., Alnassar, N., Hillman, C., Karlsson, K.Æ., Norton, W.H.J., Parker, M.O. (2023) adgrl3.1-deficient zebrafish show noradrenaline-mediated externalizing behaviors, and altered expression of externalizing disorder-candidate genes, suggesting functional targets for treatment. Translational psychiatry. 13:304304
Sveinsdóttir, H.S., Christensen, C., Þorsteinsson, H., Lavalou, P., Parker, M.O., Shkumatava, A., Norton, W.H.J., Andriambeloson, E., Wagner, S., Karlsson, K.Æ. (2022) Novel non-stimulants rescue hyperactive phenotype in an adgrl3.1 mutant zebrafish model of ADHD. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology. 48(8):1155-1163
Pérez-Rico, Y.A., Barillot, E., Shkumatava, A. (2020) Demarcation of Topologically Associating Domains Is Uncoupled from Enriched CTCF Binding in Developing Zebrafish. iScience. 23:101046
Lavalou, P., Eckert, H., Damy, L., Constanty, F., Majello, S., Bitetti, A., Graindorge, A., Shkumatava, A. (2019) Strategies for Genetic Inactivation of Long Noncoding RNAs in Zebrafish. RNA (New York, N.Y.). 25(8):897-904
Bitetti, A., Mallory, A.C., Golini, E., Carrieri, C., Carreño Gutiérrez, H., Perlas, E., Pérez-Rico, Y.A., Tocchini-Valentini, G.P., Enright, A.J., Norton, W.H.J., Mandillo, S., O'Carroll, D., Shkumatava, A. (2018) MicroRNA degradation by a conserved target RNA regulates animal behavior. Nature structural & molecular biology. 25(3):244-251
Pérez Rico, Y.A., Boeva, V., Mallory, A.C., Bitetti, A., Majello, S., Barillot, E., Shkumatava, A. (2017) Comparative analyses of super-enhancers reveal conserved elements in vertebrate genomes. Genome research. 27(2):259-268
Haeussler, M., Schönig, K., Eckert, H., Eschstruth, A., Mianné, J., Renaud, J.B., Schneider-Maunoury, S., Shkumatava, A., Teboul, L., Kent, J., Joly, J.S., Concordet, J.P. (2016) Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR. Genome biology. 17:148
Ulitsky, I., Shkumatava, A., Jan, C., Subtelny, A.O., Koppstein, D., Bell, G., Sive, H., and Bartel, D. (2012) Extensive alternative polyadenylation during zebrafish development. Genome research. 22(10):2054-2066
Ulitsky, I., Shkumatava, A., Jan, C.H., Sive, H., and Bartel, D.P. (2011) Conserved Function of lincRNAs in Vertebrate Embryonic Development despite Rapid Sequence Evolution. Cell. 147(7):1537-1550
Shkumatava, A., Stark, A., Sive, H., and Bartel, D.P. (2009) Coherent but overlapping expression of microRNAs and their targets during vertebrate development. Genes & Development. 23(4):466-481
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