ZFIN ID: ZDB-COMPANY-060421-1
Noldus Information Technology bv
Contact Person: Thoomes-Wildschut, Sandra
Email: info@noldus.com
URL: http://www.noldus.com
Address: International Headquarters P.O. Box 268 6700 AG Wageningen The Netherlands
Country: Netherlands
Phone: 1-800-355-9541
Fax: 703-771-0441
Line Designation: None assigned


PRODUCTS AND SERVICES
North American Headquarters
1503 Edwards Ferry Road
Suite 201
Leesburg, Virginia 20176
USA

Noldus Information Technology was established in 1989 and over the years has grown into the market leader in software and instruments for animal and human behavior research. Their products enable the collection, integration, analysis, management, and presentation of behavioral data, and are validated in thousands of publications worldwide. With offices and distributors around the world, Noldus strives to provide products that make optimal use of both human and animal resources, allowing researchers to use as less animals as possible to get valuable results in research.

Video analysis
EthoVision XT is video tracking software that tracks and analyzes activity and behavior of any animal in any kind of set-up. That includes zebrafish embryos, larvae and adults. It has proven its worth in thousands of publications.

Zebrafish embryo activity measurement
EthoVision XT is able to detect the activity of zebrafish embryos from a video file or live camera image, for example from a video microscope. Activity detection in EthoVision XT detects changes at pixel level from one frame to the next, effectively measuring events such as tail coilings, convulsions, and swimming bouts. Several embryos can be monitored simultaneously, making it suitable for high-throughput testing in developmental studies or toxicology.

Zebrafish larvae heartbeat monitoring
In a similar way to embryo activity measurement, EthoVision XT can monitor the heartbeat of larvae. First the heart region is specified, so that activity can be measured in this area only. The activity data is processed by the Heartbeat Detector, that extracts a power spectrum to specify the heartbeat frequency. No need for fluorescence methods or specific training of your staff.

Zebrafish larvae activity high-throughput testing
DanioVision is a system that combines the power of EthoVision XT with a completely controlled environment for testing in standard multi-well plates or petri dishes. This means you can track the activity, motility, swimming pattern, etc. of up to 96 individuals simultaneously. The built-in camera, IR backlight, and innovative design gives EthoVision XT a perfect image for tracking. You can control the light conditions, e.g. to study circadian rhythmicity or use light as a sudden stimulus, and you can control the temperature with a water flow-through system.

Zebrafish adult behavior
Zebrafish adults show similar behavioral endpoints to rodents, and many traditional rodents test have been successfully translated in pharmacological research and studies on genetics. Examples include cross-maze, T-maze, novel tank diving (bottom dwelling), light-dark boxes, social preference, and social interaction studies. EthoVision XT is able to accurately track from a video image from above (e.g. T-maze) or from a side view (e.g. tank diving). You can track multiple fish together, and automatically determine variables such as shoaling, which is traditionally a labor-intensive procedure. You can also combine other hardware in your set-up, such as screens that play video images next to the aquaria or automated feeders.

DanioScope
DanioScope is a new software tool to investigate a scope of zebrafish embryo and larvae parameters. It includes features to measure embryo activity, cardiovascular measurements, morphology, and gut and blood flow. Measurements are based on video files and images, offering an easy-to-use and non-invasive way to study your zebrafish. Most importantly, DanioScope allows you to test many animals simultaneously, increasing the throughput of your research significantly, and ultimately saving you a lot of time when it comes to preparing your experiment, acquiring data, and analyzing the results. Moreover, automating your research with DanioScope means using a consistent measuring tool – eliminating inter- and intra-observer variability.

Three-dimensional tracking
Some research needs more detail, for example to detect subtle differences in swimming patterns. Because zebrafish swim in three dimensions, not two, 3D tracking can offer the spatiotemporal data to acquire this level of detail. Track3D is a system that tracks in 3D and visualizes the trajectory in a 3D image.


COMPANY REPRESENTATIVES
Noldus, Lucas Executive Thoomes-Wildschut, Sandra Marketing/Product Dev.

ZEBRAFISH PUBLICATIONS OF COMPANY REPRESENTATIVES
Stewart, A.M., Grieco, F., Tegelenbosch, R.A., Kyzar, E.J., Nguyen, M., Kaluyeva, A., Song, C., Noldus, L.P., Kalueff, A.V. (2015) A novel 3D method of locomotor analysis in adult zebrafish: Implications for automated detection of CNS drug-evoked phenotypes. Journal of Neuroscience Methods. 255:66-74
Green, J., Collins, C., Kyzar, E., Pham, M., Roth, A., Gaikwad, S., Cachat, J., Stewart, A.M., Landsman, S., Grieco, F., Tegelenbosch, R., Noldus, L., and Kalueff, A.V. (2012) Automated high-throughput neurophenotyping of zebrafish social behavior. Journal of Neuroscience Methods. 210(2):266-271