ZFIN Company: Phylonix Pharmaceuticals, Inc.

ZFIN ID: ZDB-COMPANY-011019-1

Phylonix Pharmaceuticals, Inc.

Contact Person:	Trowbridge, Stephanie
Email:	stephanie@phylonix.com
URL:	http://www.Phylonix.com
Address:	Suite 300 100 Inman Street Cambridge, MA 02139 USA
Country:	United States
Phone:	(617) 441-6700 ext. 16
Fax:	(617) 441-6766
Line Designation:	None assigned

PRODUCTS AND SERVICES

Phylonix Pharmaceuticals, Inc. is a developmental biology company focused on developing and marketing novel in vivo zebrafish based assays for therapeutic and industrial screening for cancers, central nervous system and cardiovascular diseases, apoptosis, and organ toxicity. The company is assembling embryo and chemical sample handling instrumentation to automate analysis. The company has established a state of the art aquaculture facility.

COMPANY REPRESENTATIVES

Trowbridge, Stephanie Administrative	McGrath, Patricia Executive	Parng, Chuenlei Technical
Willett, Catherine Technical

ZEBRAFISH PUBLICATIONS OF COMPANY REPRESENTATIVES

McGrath, P., and Seng, W.L. (2013) Use of zebrafish apoptosis assays for preclinical drug discovery. Expert opinion on drug discovery. 8(10):1191-202

McGrath, P., Seng, W.L., Willett, C., and Augustine, K.A. (2008) Determination of LD50 and assessment of drug induced developmental toxicity in zebrafish. Journal of Pharmacological and Toxicological Methods. 58(2):150

McGrath, P., and Li, C.Q. (2008) Zebrafish: a predictive model for assessing drug-induced toxicity. Drug discovery today. 13(9-10):394-401

Parng, C., Roy, N.M., Ton, C., Lin, Y., and McGrath, P. (2007) Neurotoxicity assessment using zebrafish. Journal of Pharmacological and Toxicological Methods. 55(1):103-112

Parng, C., Ton, C., Lin, Y.X., Roy, N.M., and McGrath, P. (2006) A zebrafish assay for identifying neuroprotectants in vivo. Neurotoxicology and teratology. 28(4):509-516

Ton, C., Lin, Y., and Willett, C. (2006) Zebrafish as a model for developmental neurotoxicity testing. Birth defects research. Part A, Clinical and molecular teratology. 76(7):553-567

Haldi, M., Ton, C., Seng, W.L., and McGrath, P. (2006) Human melanoma cells transplanted into zebrafish proliferate, migrate, produce melanin, form masses and stimulate angiogenesis in zebrafish. Angiogenesis. 9(3):139-151

Ton, C., and Parng, C. (2005) The use of zebrafish for assessing ototoxic and otoprotective agents. Hearing Research. 208(1-2):79-88

Parng, C. (2005) In vivo zebrafish assays for toxicity testing. Curr. Opin. Drug Discov. Devel.. 8(1):100-106

Danilova, N., Visel, A., Willett, C.E., and Steiner, L.A. (2004) Expression of the winged helix/forkhead gene, foxn4, during zebrafish development. Dev. Brain Res.. 153(1):115-119

Danilova, N., Hohman, V.S., Sacher, F., Ota, T., Willett, C.E., and Steiner, L.A. (2004) T cells and the thymus in developing zebrafish. Developmental and comparative immunology. 28(7-8):755-767

Parng, C., Anderson, N., Ton, C., and McGrath, P. (2004) Zebrafish apoptosis assays for drug discovery. The Zebrafish: Cellular and Developmental Biology,2nd Ed. Methods Cell Biol.. 76:75-85

Seng, W.L., Eng, K., Lee, J., and McGrath, P. (2004) Use of a monoclonal antibody specific for activated endothelial cells to quantitate angiogenesis in vivo in zebrafish after drug treatment. Angiogenesis. 7(3):243-253

Steiner, L.A., Willett, C.E., and Danilova, N. (2004) The immune system in zebrafish. In Biology of B Cells, 3rd edition, Alt, F.W., Honjo, T., and Neuberger, M.S., eds., in press.

Ma, C., Parng, C.L., Seng, W.L., Zhang, C., Willett, C., and McGrath, P. (2003) Zebrafish – An in vivo model for drug screening. Innovations in Pharmaceutical Technologies. Nov. 2003:38-45

Zhang, C., Willett, C., and Fremgen, T. (2003) Zebrafish: An animal model for toxicological studies. Current Protocols in Toxicology. Unit 1.7

Parng, C., Seng, W.L., Semino, C., and McGrath, P. (2002) Zebrafish: a preclinical model for drug screening. Assay and drug development technologies. 1(1 Pt 1):41-8

Willett, C.E., Kawasaki, H., Amemiya, C.T., Lin, S., and Steiner, L.A. (2001) Ikaros expression as a marker for lymphoid progenitors during zebrafish development. Developmental Dynamics : an official publication of the American Association of Anatomists. 222(4):694-698

Jessen, J.R., Willett, C.E., and Lin, S. (1999) Artificial chromosome transgenesis reveals long-distance negative regulation of rag1 in zebrafish. Nature Genetics. 23(1):15-16

Willett, C.E., Cortes, A., Zuasti, A., and Zapata, A.G. (1999) Early hematopoiesis and developing lymphoid organs in the zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 214:323-336

Serbedzija, G.N., Flynn, E., and Willett, C.E. (1999) Zebrafish angiogenesis: A new model for drug screening. Angiogenesis. 3(4):353-359

Bertrand, F.E., III, Olson, S.L., Willett, C.E., and Wu, G.E. (1998) Sequence of the RAG1 and RAG2 Intergenic Region in Zebrafish (Danio rerio). Developmental immunology. 5:211-214

Willett, C.E., Zapata, A.G., Hopkins, N.A., and Steiner, L.A. (1997) Expression of zebrafish rag genes during early development identifies the thymus. Developmental Biology. 182(2):331-341

Willett, C.E., Cherry, J.J., and Steiner, L.A. (1997) Characterization and expression of the recombination activating genes (rag1 and rag2) of zebrafish. Immunogenetics. 45(6):394-404