Inhibition of zebrafish Zcchc6/11 genes results in improper Hox expression and embryonic development. (a) The zcchc6 or zcchc11 morphant embryos display multiple defects at 48 hpf, including degeneration of somites, failure of tail elongation, pericardial edema and developmental delay. RT-PCR was used to measure the relative knockdown of Zcchc6 and Zcchc11. PCR products were resolved using agarose gel electrophoresis and visualized by ethidium bromide staining (b) Quantitative PCR analysis (on 24-h embryos) shows the reduction of several Hox genes. Blue bars are control and red bars represent Zcchc11 MO (candd) Hoxb8b was reduced in Zcchc6 and Zcchc11 morphant embryos at 24 hpf. The numbers indicated in the right hand corner of each picture: the number of affected embryos similar to what is shown (left) and the total number of observed embryos (right).
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|Acknowledgments:||ZFIN wishes to thank the journal Nucleic acids research for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nucleic Acids Res.|