Lateral and dorsal views showing the expression of drd1 in developing embryos at 30 hours postfertilization (hpf) (A,C,E) and 36 hpf (B,D,F). Anterior is to the left. During early development, the expression of drd1 was detected in the diencephalon, rhombomeres, and infundibulum. Scale bar = 100 μm in A,B, 30 μm in C-F.

Expression of drd1 in developing embryos. A-C: Lateral and dorsal views of developing embryos at 48 hours postfertilization (hpf) and 120 hpf. Anterior is to the left. Note the increase of drd1 expression in the late developmental stages. D: Expression of drd1 in the retina at 120 hpf. Expression of drd1 was detected throughout the entire inner nuclear layer with the exception of the marginal zones (outlined by dashed lines). ONL, outer nuclear layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bar = 70 μm in A-C, 35 μm in D.

In situ hybridization of drd1 in adult zebrafish retinas (6 months) using antisense (A) or sense (B) probes. The expression of drd1 was detected in both the inner and outer nuclear layers as well as the ganglion cell layer. RPE, retinal pigment epithelia; ONL, outer nuclear layer; INL, inner nuclear layer, IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bar = 200 μm.