Transgenic Construct

Tg(GFP,cryaa:mVenus)

ID
ZDB-TGCONSTRCT-240313-1
Name
Tg(GFP,cryaa:mVenus)
Previous Names
  • Tg(GFP-Sara) (1)
Type
engineered_plasmid
Regulatory Regions
Coding Sequences
Contains Sequences
None
Note
sequencing of GFP insertion with the following set of primers: forward ATCCTGCACTGAATGCACAC and backward CATGCTCGAGGAGAATTGGTGTGTCCGTTTC. Also, a sequence coding for mVenus under the expression of the Cryaa promoter was inserted in the intron 3 of the zfyve9a gene. You can track mVenus expression in the retina after 2 dpf to select positives embryos. GFP-zfyve9a is barely detectable under a fluorescent binocular. Insertion of GFP in front of the exon 2 of zfyve9a gene
Plasmid Map
None
Genomic Features
That Utilize tg(gfp,cryaa:mvenus) Construct
Genomic Feature Affected Genomic Regions
ug111Tg zfyve9a
1 - 1 of 1
Transgenics
That Utilize tg(gfp,cryaa:mvenus) Construct
No data available
Sequence Information
Other Construct Pages
None
Citations