Transgenic Construct

Tg(BMPRE-AAV.Mlp:mKOFP2)

ID

ZDB-TGCONSTRCT-110310-2

Previous Names

Tg(BMPRE-AAV.Mlp:mKO2)
Tg(BmpRE-AAVmlp:mKO2)
Tg(BRE-AAVmlp:dmKO2) (1)
Tg(Mmu.Id1-AAVmlp:mKO2) (1)

Type

engineered_plasmid

Regulatory Regions

None

Coding Sequences

mKOFP2 (1)

[Em λ][Ex λ]

Fpbase:mKO2

Contains Sequences

BMPRE (1)

Note

This construct utilizes the 84bp BMP Response Element (BRE) derived from mouse Id1 promoter, containing multiple short Smad-binding sites, and the Adeno-associated virus (AAV) major late promoter (mlp) driving expression of destabilized mKO2 with a C-terminal PEST domain from zebrafish Notch3 to facilitate rapid degradation by means of ubiquitinylation.

Plasmid Map

None

Genomic Features

That Utilize Tg(BMPRE-AAV.Mlp:mKOFP2) Construct

Genomic Feature	Affected Genomic Regions
mw40Tg

Transgenics

That Utilize Tg(BMPRE-AAV.Mlp:mKOFP2) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
bmp4^st72/st72; smad5^b1100/b1100; mw40Tg/mw40Tg	bmp4 smad5
bmp4^st72/st72; smad5^b1100/b1100; mw40Tg/mw40Tg; s870Tg/s870Tg	bmp4 smad5	Fig. 6. from Klem et al., 2025
mw40Tg			3 figures from 2 publications
mw40Tg/+ (AB)
mw40Tg/mw40Tg; s870Tg/s870Tg		Fig. 6. from Klem et al., 2025

Sequence Information

Other Construct Pages

None

Citations