Transgenic Construct

Tg4(Xla.Rho:crb2a-HA)

ID

ZDB-TGCONSTRCT-110131-39

Previous Names

Intra deltaFBD (1)
Tg(Xla.Rho:crb2.4-HA) (1)
Tg(Xla.Rho:crb2a.4-HA)

Type

engineered_plasmid

Regulatory Regions

None

Coding Sequences

crb2a (1)
HA (1)

Contains Sequences

HA (1)

Note

This construct utilizes 0.8 kB of the Xenopus rod opsin (Rho) promoter with an N terminal signal peptide taken from zebrafish Crb2b and an influenza hamagglutinin (HA) tag (YPYDVPDYA). To make the Intra deltaFBD construct, the conserved amino acids tyrosine (T10) and glutamic acid (E16) of IntraWT construct were substituted with alanines to compromise the FERM-binding domain.

Plasmid Map

None

Genomic Features

That Utilize Tg4(Xla.Rho:crb2a-HA) Construct

Genomic Feature	Affected Genomic Regions
umz13Tg
umz14Tg
umz15Tg

Transgenics

That Utilize Tg4(Xla.Rho:crb2a-HA) Construct

Fish	Affected Genomic Regions	Gene Expression
slc45a2^b4/+; fl1Tg/+; umz13Tg/+	slc45a2
slc45a2^b4/+; fl1Tg/+; umz14Tg/+	slc45a2
slc45a2^b4/b4; fl1Tg; umz13Tg	slc45a2	5 figures from Hsu et al., 2010
slc45a2^b4/b4; fl1Tg; umz14Tg	slc45a2
slc45a2^b4/b4; fl1Tg; umz15Tg	slc45a2
umz15Tg

Sequence Information

Other Construct Pages

None

Citations