PUBLICATION

Anterior movement of ventral diencephalic precursors separates the primordial eye field in the neural plate and requires cyclops

Authors
Varga, Z.M., Wegner, J., and Westerfield, M.
ID
ZDB-PUB-991201-72
Date
1999
Source
Development (Cambridge, England)   126: 5533-5546 (Journal)
Registered Authors
Varga, Zoltan M., Wegner, Jeremy, Westerfield, Monte
Keywords
fate map; anterior neural plate; cyclopia; holoprosencephaly; zebrafish; Danio rerio; gene expression; morphogenesis; odd-paired-like (opl)
MeSH Terms
  • Animals
  • Diencephalon/embryology*
  • Eye/embryology*
  • Gastrula
  • Gene Expression Regulation, Developmental
  • Mutation
  • Retina/embryology
  • Stem Cells
  • Zebrafish/embryology
PubMed
10572031
Abstract
A currently favored hypothesis postulates that a single field of cells in the neural plate forms bilateral retinas. To learn how retinal precursors segregate, we followed individual labeled neural plate cells in zebrafish. In the late gastrula, a single field of odd-paired-like-expressing cells contributed to both retinas, bordered posteriorly by diencephalic precursors expressing mariposa. Median mariposa-expressing cells moved anteriorly, separating the eyes, and formed ventral anterior diencephalon, the presumptive hypothalamus. In cyclops mutants, corresponding cells failed to move anteriorly, a ventral diencephalon never formed, and the eyes remained fused. Ablation of the region containing these cells induced cyclopia in wild types. Our results indicate that movement of a median subpopulation of diencephalic precursors separates retinal precursors into left and right eyes. Wild-type cyclops gene function is required for these morphogenetic movements.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes