PUBLICATION
Cloning, sequencing and differential expression of alphaB-crystallin in the zebrafish, Danio rerio
- Authors
- Posner, M., Kantorow, M., and Horwitz, J.
- ID
- ZDB-PUB-991112-6
- Date
- 1999
- Source
- BBA Gene Structure and Expression 1447(2-3): 271-277 (Journal)
- Registered Authors
- Posner, Mason
- Keywords
- alpha B-crystallin; lens; RT-PCR; small heat shock protein; zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- Crystallins/genetics*
- Gene Expression Regulation*
- Molecular Sequence Data
- Sequence Alignment
- Sequence Analysis
- Zebrafish
- PubMed
- 10542326 Full text @ BBA Gene Structure and Expression
Citation
Posner, M., Kantorow, M., and Horwitz, J. (1999) Cloning, sequencing and differential expression of alphaB-crystallin in the zebrafish, Danio rerio. BBA Gene Structure and Expression. 1447(2-3):271-277.
Abstract
Here we report the cloning and expression of alphaB-crystallin from the zebrafish. 5'- and 3'-RACE was used to isolate a 900-bp transcript that contained insertions and deletions that differentiate it from both alphaA-crystallin and HSP-27. The deduced amino acid sequence of zebrafish alphaB-crystallin revealed that it lacked four residues in the C-terminus implicated in protein-protein interactions in other vertebrate species. In addition, the sequence contained two substitutions at sites implicated in phosphorylation in other vertebrate species. Northern analysis and semi-quantitative RT-PCR indicate that zebrafish alphaB-crystallin is expressed at extremely low levels outside of the lens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping