PUBLICATION
Transient establishment of anteroposterior polarity in the zebrafish pectoral fin bud in the absence of sonic hedgehog activity
- Authors
- Neumann, C.J., Grandel, H., Gaffield, W., Schulte-Merker, S., Nüsslein-Volhard, C.
- ID
- ZDB-PUB-991103-2
- Date
- 1999
- Source
- Development (Cambridge, England) 126(21): 4817-4826 (Journal)
- Registered Authors
- Grandel, Heiner, Neumann, Carl J., Nüsslein-Volhard, Christiane, Schulte-Merker, Stefan
- Keywords
- sonic hedgehog; limb; fin; ZPA; hox; colinearity; cyclopamine; retinoic acid; zebrafish; Danio rerio
- MeSH Terms
-
- Animals
- Body Patterning/physiology*
- Bone Morphogenetic Protein 2
- Bone Morphogenetic Proteins/genetics
- Cell Division/genetics
- DNA-Binding Proteins/genetics
- Ectoderm
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/drug effects
- Gene Expression Regulation, Developmental
- Hedgehog Proteins
- Homeodomain Proteins/genetics
- Larva
- Limb Buds/cytology
- Limb Buds/growth & development*
- Membrane Proteins/genetics
- Mutation
- Proteins/genetics*
- Proteins/metabolism
- Receptors, Cell Surface
- Trans-Activators*
- Transcription Factors/genetics
- Transforming Growth Factor beta*
- Tretinoin/pharmacology
- Veratrum Alkaloids/pharmacology
- Zebrafish/embryology*
- Zebrafish Proteins*
- PubMed
- 10518498 Full text @ Development
Citation
Neumann, C.J., Grandel, H., Gaffield, W., Schulte-Merker, S., Nüsslein-Volhard, C. (1999) Transient establishment of anteroposterior polarity in the zebrafish pectoral fin bud in the absence of sonic hedgehog activity. Development (Cambridge, England). 126(21):4817-4826.
Abstract
Sonic hedgehog (Shh) is expressed in the posterior vertebrate limb bud mesenchyme and directs anteroposterior patterning and growth during limb development. Here we report an analysis of the pectoral fin phenotype of zebrafish sonic you mutants, which disrupt the shh gene. We show that Shh is required for the establishment of some aspects of anteroposterior polarity, while other aspects of anteroposterior polarity are established independently of Shh, and only later come to depend on Shh for their maintenance. We also demonstrate that Shh is required for the activation of posterior HoxD genes by retinoic acid. Finally, we show that Shh is required for normal development of the apical ectodermal fold, for growth of the fin bud, and for formation of the fin endoskeleton.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping