ZFIN ID: ZDB-PUB-990727-17
Zebrafish stat3 is expressed in restricted tissues during embryogenesis and stat1 rescues cytokine signaling in a STAT1-deficient human cell line
Oates, A.C., Wollberg, P., Pratt, S.J., Paw, B.H., Johnson, S.L., Ho, R.K., Postlethwait, J.H., Zon, L.I., and Wilks, A.F.
Date: 1999
Source: Developmental dynamics : an official publication of the American Association of Anatomists   215(4): 352-370 (Journal)
Registered Authors: Oates, Andrew, Paw, Barry, Zon, Leonard I.
Keywords: zebrafish; stat1, stat3, STAT, JAK; cytokine; interferon-alpha, interferon-gamma; IL-6, slL6; signal transduction; phosphotyrosine; transcription factor; development; embryogenesis; blastula; prechordal plate; cranial neural crest; cranial motor nuclei, sensory ganglia; lateral line; ganglion cell; synteny; degenerate oligonucleotide PCR; gene mapping
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Central Nervous System/metabolism
  • Chromosome Mapping
  • Chromosomes
  • Conserved Sequence
  • DNA-Binding Proteins/analysis
  • DNA-Binding Proteins/genetics*
  • DNA-Binding Proteins/metabolism*
  • Embryo, Nonmammalian/anatomy & histology
  • Evolution, Molecular
  • Humans
  • Janus Kinase 1
  • Models, Genetic
  • Molecular Sequence Data
  • Multigene Family
  • Peripheral Nervous System/metabolism
  • Phylogeny
  • Polymorphism, Genetic
  • Protein-Tyrosine Kinases/metabolism
  • STAT1 Transcription Factor
  • STAT3 Transcription Factor
  • Sequence Homology, Amino Acid
  • Time Factors
  • Tissue Distribution
  • Trans-Activators/analysis
  • Trans-Activators/genetics*
  • Trans-Activators/metabolism*
  • Transfection
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins
PubMed: 10417824 Full text @ Dev. Dyn.
Transcription factors of the STAT family are required for cellular responses to multiple signaling molecules. After ligand binding-induced activation of cognate receptors, STAT proteins are phosphorylated, hetero- or homodimerize, and translocate to the nucleus. Subsequent STAT binding to specific DNA elements in the promoters of signal-responsive genes alters the transcriptional activity of these loci. STAT function has been implicated in the transduction of signals for growth, reproduction, viral defense, and immune regulation. We have isolated and characterized two STAT homologs from the zebrafish Danio rerio. The stat3 gene is expressed in a tissue-restricted manner during embryogenesis, and larval development with highest levels of transcript are detected in the anterior hypoblast, eyes, cranial sensory ganglia, gut, pharyngeal arches, cranial motor nuclei, and lateral line system. In contrast, the stat1 gene is not expressed during early development. The stat3 gene maps to a chromosomal position syntenic with the mouse and human STAT3 homologs, whereas the stat1 gene does not. Despite a higher rate of evolutionary change in stat1 relative to stat3, the stat1 protein rescues interferon-signaling functions in a STAT1-deficient human cell line, indicating that cytokine-signaling mechanisms are likely to be conserved between fish and tetrapods.