PUBLICATION
R-cadherin expression in the developing and adult zebrafish visual system
- Authors
- Liu, Q., Sanborn, K.L., Cobb, N., Raymond, P.A., and Marrs, J.A.
- ID
- ZDB-PUB-990720-21
- Date
- 1999
- Source
- The Journal of comparative neurology 410(2): 303-319 (Journal)
- Registered Authors
- Keywords
- optic tectum; retinal ganglion cells; development; cell adhesion
- MeSH Terms
-
- Age Factors
- Amino Acid Sequence
- Animals
- Cadherins/genetics*
- Cadherins/metabolism*
- Cell Adhesion Molecules/genetics
- Cell Adhesion Molecules/metabolism
- DNA, Complementary/genetics
- Diencephalon/cytology
- Diencephalon/embryology
- Diencephalon/metabolism
- Gene Expression/genetics
- Gene Library
- Immunoblotting
- In Situ Hybridization
- Molecular Sequence Data
- Retinal Ganglion Cells/cytology
- Retinal Ganglion Cells/metabolism*
- Superior Colliculi/cytology
- Superior Colliculi/metabolism
- Visual Pathways/embryology
- Visual Pathways/growth & development
- Visual Pathways/metabolism*
- Zebrafish/embryology
- Zebrafish/genetics
- PubMed
- 10414535 Full text @ J. Comp. Neurol.
Citation
Liu, Q., Sanborn, K.L., Cobb, N., Raymond, P.A., and Marrs, J.A. (1999) R-cadherin expression in the developing and adult zebrafish visual system. The Journal of comparative neurology. 410(2):303-319.
Abstract
Cell adhesion molecules in the cadherin family have been implicated in histogenesis and maintenance of cellular structure and function in several organs. Zebrafish have emerged as an important new developmental model, but only three zebrafish cadherin molecules have been identified to date (N-cadherin, paraxial protocadherin, and VN-cadherin). We began a systematic study to identify other zebrafish cadherins by screening zebrafish cDNA libraries using an antibody raised to the cytoplasmic domain of mouse E-cadherin. Here, we report a partial cDNA with extensive sequence homology to R-cadherin. Spatial and temporal expression of this putative zebrafish R-cadherin was examined in embryos and adults by Northern analysis, RNase protection, and in situ hybridization. R-cadherin message increased during embryogenesis up to 80 hours postfertilization (hpf) and persisted in adults. In the embryonic brain, R-cadherin was first expressed in groups of cells in the diencephalon and pretectum. In adult zebrafish brain, R-cadherin continued to be expressed in several specific regions including primary visual targets. In the retina, R-cadherin was first detected at about 33 hours postfertilization in the retinal ganglion cell layer and the inner part of the inner nuclear layer. Expression levels were highest during periods of axon outgrowth and synaptogenesis. Retrograde labeling of the optic nerve with 1,1'-dioctadecyl-3,3,3',3', tetramethylindocarbocyanine perchlorate (DiI) followed by in situ hybridization confirmed that a subset of retinal ganglion cells in the embryo expressed R-cadherin message. In the adult, R-cadherin expression continued in a subpopulation of retinal ganglion cells. These results suggest that R-cadherin-mediated adhesion plays a role in development and maintenance of neuronal connections in zebrafish visual system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping