PUBLICATION
The effects of temperature on the dark-adapted spectral sensitivity function of the adult zebrafish
- Authors
- Saszik, S. and Bilotta, J.
- ID
- ZDB-PUB-990405-9
- Date
- 1999
- Source
- Vision Research 39: 1051-1058 (Journal)
- Registered Authors
- Bilotta, Joe
- Keywords
- Danio rerio; dark-adapted spectral sensitivity; electroretinogram; temperature
- MeSH Terms
-
- Adaptation, Ocular/physiology*
- Animals
- Electroretinography
- Female
- Male
- Photic Stimulation
- Retinal Cone Photoreceptor Cells/physiology
- Retinal Pigments/physiology*
- Retinal Rod Photoreceptor Cells/physiology
- Rhodopsin/physiology
- Temperature*
- Zebrafish/physiology*
- PubMed
- 10343824 Full text @ Vision Res.
Citation
Saszik, S. and Bilotta, J. (1999) The effects of temperature on the dark-adapted spectral sensitivity function of the adult zebrafish. Vision Research. 39:1051-1058.
Abstract
In goldfish and other cold-blooded vertebrates, temperature can influence the rhodopsin/porphyropsin contributions to the rod photoreceptors. This study examined the effects of temperature on the spectral sensitivity function of the dark-adapted zebrafish. Zebrafish were housed in either a warm (28-30 degrees C) or cold (22-25 degrees C) tank prior to testing. Fish were dark-adapted for at least 1 h and electroretinogram (ERG) responses to 200 ms stimuli of various wavelengths and irradiances were obtained. Results show that water temperature affected the spectral sensitivity function of the ERG b-wave. Subjects housed in the warm temperatures had a spectral sensitivity consistent with the rhodopsin absorption curve; however, fish housed in the colder temperatures had a spectral sensitivity function that was the result of a rhodopsin/porphyropsin mixture. In addition, ultraviolet cones (lambda max: 362 nm) contributed to the dark-adapted spectral sensitivity function under both temperature conditions. Consistent with the results from other fish, the dark-adapted visual system of the zebrafish can be influenced by water temperature. The results of this study demonstrate the necessity of maintaining a stable environment when examining the contributions of the photoreceptors to the visual response.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping