PUBLICATION
Production of androgenetic haploids in zebrafish with ultraviolet light
- Authors
- Ungar, A.R., Helde, K.A., and Moon, R.T.
- ID
- ZDB-PUB-990316-4
- Date
- 1998
- Source
- Molecular marine biology and biotechnology 7: 320-326 (Journal)
- Registered Authors
- Helde, Kathryn, Moon, Randall T., Ungar, Anne
- Keywords
- Animal; Diploidy; Embryo, Nonmammalian; Female; Genomic Imprinting; Haploidy; Male; Oocytes; Polymerase Chain Reaction; Sperm-Ovum Interactions; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Ultraviolet Rays; Zebrafish
- MeSH Terms
-
- Animals
- Diploidy
- Embryo, Nonmammalian/physiology
- Embryo, Nonmammalian/radiation effects*
- Female
- Genomic Imprinting
- Haploidy*
- Male
- Oocytes/radiation effects*
- Polymerase Chain Reaction/methods
- Sperm-Ovum Interactions
- Ultraviolet Rays*
- Zebrafish/genetics*
- PubMed
- 9892723
Citation
Ungar, A.R., Helde, K.A., and Moon, R.T. (1998) Production of androgenetic haploids in zebrafish with ultraviolet light. Molecular marine biology and biotechnology. 7:320-326.
Abstract
We describe a simple method for producing androgenetic haploid embryos in zebrafish. Mature eggs are expressed, and the maternal genome is inactivated by irradiation with short-wave ultraviolet light prior to in vitro fertilization. We demonstrate the absence of maternal genome in the resultant embryos using assays based on morphology and polymerase chain reaction. This technique for the production of androgenetic haploids will be extremely useful for identifying and recovering mutations as a complement to, or in place of, generating gynogenetic haploids.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping