PUBLICATION
Characterisation of a second patched gene in the zebrafish Danio rerio and the differential response of patched genes to hedgehog signalling
- Authors
- Lewis, K.E., Concordet, J.P., and Ingham, P.W.
- ID
- ZDB-PUB-990315-8
- Date
- 1999
- Source
- Developmental Biology 208: 14-29 (Journal)
- Registered Authors
- Ingham, Philip, Lewis, Katharine E.
- Keywords
- hedgehog; signalling; patched2; receptor; zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- Embryonic Development
- Gene Expression Regulation, Developmental/genetics
- Hedgehog Proteins
- Membrane Proteins/chemistry
- Membrane Proteins/genetics*
- Microinjections
- Molecular Sequence Data
- Mutation
- Phylogeny
- Proteins/genetics*
- RNA, Antisense/genetics
- RNA, Messenger/metabolism
- Receptors, Cell Surface
- Sequence Alignment
- Sequence Homology, Amino Acid
- Signal Transduction/genetics
- Trans-Activators*
- Transcription, Genetic/genetics
- Zebrafish/genetics*
- Zebrafish Proteins*
- PubMed
- 10075838 Full text @ Dev. Biol.
Citation
Lewis, K.E., Concordet, J.P., and Ingham, P.W. (1999) Characterisation of a second patched gene in the zebrafish Danio rerio and the differential response of patched genes to hedgehog signalling. Developmental Biology. 208:14-29.
Abstract
Hedgehog signalling has been implicated in a variety of processes in vertebrate development, and in each case, the activity of Hh proteins is thought to be mediated by their interaction with a large multipass transmembrane protein encoded by the patched (ptc) gene. In this study we present the full-length coding sequence and describe the wild-type expression pattern of a second ptc gene in zebrafish, Zf-ptc2. We find that at the sequence level Zf-ptc2 is more closely related than Zf-ptc1 to the ptc genes initially characterised in other vertebrate species. We also show that transcription of Zf-ptc2, like Zf-ptc1, is dependent upon Hh signalling and present evidence that it is activated in response to lower levels of Hh activity than is Zf-ptc1. In addition we find no evidence for any specificity in the regulatory interactions between the various Hh proteins and the two ptc genes in the zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping