ZFIN ID: ZDB-PUB-990119-21
Analysis of zebrafish semaphorin reveals potential functions in vivo
Halloran, M.C., Severance, S.M., Yee, C.S., Gemza, D.L., Raper, J.A., and Kuwada, J.Y.
Date: 1999
Source: Developmental dynamics : an official publication of the American Association of Anatomists   214: 13-25 (Journal)
Registered Authors: Halloran, Mary, Kuwada, John
Keywords: axon guidance; semaphorin; collapsin; zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Axons/physiology
  • Branchial Region/embryology
  • Central Nervous System/embryology
  • Chickens
  • Gene Expression Regulation, Developmental
  • Mesencephalon/embryology
  • Molecular Sequence Data
  • Mutation
  • Nerve Growth Factors/genetics
  • Nerve Growth Factors/physiology*
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/physiology*
  • Neural Crest/embryology
  • Notochord/embryology
  • Prosencephalon/embryology
  • RNA, Messenger
  • Rhombencephalon/embryology
  • Semaphorins
  • Sequence Homology, Amino Acid
  • Spinal Cord/embryology
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/physiology
  • Zebrafish Proteins*
PubMed: 9915572 Full text @ Dev. Dyn.
The semaphorin/collapsin gene family is a large and diverse family encoding both secreted and transmembrane proteins, some of which are thought to act as repulsive axon guidance molecules. However, the function of most semaphorins is still unknown. We have cloned and characterized several semaphorins in the zebrafish in order to assess their in vivo function. Zebrafish semaZ2 is expressed in a dynamic and restricted pattern during the period of axon outgrowth that indicates potential roles in the guidance of several axon pathways. Analysis of mutant zebrafish with reduced semaZ2 expression reveals axon pathfinding errors that implicate SemaZ2 in normal guidance.