PUBLICATION
Function of zebrafish ß-catenin and TCF-3 in dorsoventral patterning
- Authors
- Pelegri, F. and Maischein, H.M.
- ID
- ZDB-PUB-990107-8
- Date
- 1998
- Source
- Mechanisms of Development 77: 63-74 (Journal)
- Registered Authors
- Maischein, Hans-Martin, Pelegri, Francisco
- Keywords
- Amino Acid Sequence; Animal; Binding Sites; Body Patterning; Cloning, Molecular; Cytoskeletal Proteins; Embryo, Nonmammalian; Gastrula; Gene Expression Regulation, Developmental; Genes, Structural; Homeodomain Proteins; Molecular Sequence Data; Mutation; Sequence Homology, Amino Acid; Support, Non-U.S. Gov't; Transcription Factors; Zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Bacterial Proteins*
- Binding Sites/genetics
- Body Patterning/genetics
- Body Patterning/physiology*
- Cloning, Molecular
- Cytoskeletal Proteins/physiology*
- Drosophila Proteins*
- Embryo, Nonmammalian/metabolism
- Embryonic Development
- Gastrula/metabolism
- Gene Expression Regulation, Developmental
- Genes/genetics
- HMGB Proteins*
- Homeodomain Proteins/genetics
- Molecular Sequence Data
- Mutation
- Sequence Homology, Amino Acid
- TCF Transcription Factors
- Trans-Activators*
- Transcription Factor 7-Like 1 Protein
- Transcription Factors/genetics
- Transcription Factors/physiology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins
- beta Catenin
- PubMed
- 9784608 Full text @ Mech. Dev.
Citation
Pelegri, F. and Maischein, H.M. (1998) Function of zebrafish ß-catenin and TCF-3 in dorsoventral patterning. Mechanisms of Development. 77:63-74.
Abstract
We report the molecular cloning and expression of the zebrafish tcf-3 homologue and study its function and that of zebrafish betacat in dorsoventral patterning. Overexpression of mutant zTcf-3 products and Cadherin leads to a reduction in the expression of the dorsal-specific genes goosecoid and chording at the blastula stages, indicating a conserved role for betacat and tcf-3 in zebrafish dorsal axis induction. Later during gastrulation, overexpression of these same products leads to the ectopic expression of dorsal-specific genes in the marginal zone and the induction of ectopic axes, suggesting an additional role for betacat and Tcf-3 at these later stages in the repression of dorsal fates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping