PUBLICATION
Isolation, expression and regulation of a zebrafish paraxis homologue
- Authors
- Shanmugalingam, S. and Wilson, S.W.
- ID
- ZDB-PUB-990106-18
- Date
- 1998
- Source
- Mechanisms of Development 78: 85-89 (Journal)
- Registered Authors
- Shanmugalingam, Shantha, Wilson, Steve
- Keywords
- somite; spadetail; cyclops; notail; one-eyed-pinhead
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Basic Helix-Loop-Helix Transcription Factors
- DNA-Binding Proteins/biosynthesis
- DNA-Binding Proteins/genetics*
- DNA-Binding Proteins/isolation & purification
- Gastrula/metabolism
- Helix-Loop-Helix Motifs/genetics*
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism
- Homeodomain Proteins/physiology
- Humans
- Mesoderm/metabolism
- Molecular Sequence Data
- Morphogenesis/genetics
- Nuclear Proteins
- Sequence Alignment
- Sequence Homology, Amino Acid
- Somites/metabolism*
- Species Specificity
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Transcription Factors/physiology
- Vertebrates/metabolism
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish Proteins*
- PubMed
- 9858695 Full text @ Mech. Dev.
Citation
Shanmugalingam, S. and Wilson, S.W. (1998) Isolation, expression and regulation of a zebrafish paraxis homologue. Mechanisms of Development. 78:85-89.
Abstract
The formation of somites involves the subdivision of segmented presomitic mesoderm into segmentally arranged somite blocks. In mice and chicks, the basic-helix-loop-helix (bHLH) gene, paraxis, is involved in this process. Here, we report the isolation of a zebrafish homologue of paraxis, par1. par1 is expressed in presomitic paraxial mesoderm from late gastrula stages, and expression is maintained in ventrolateral cells after somite formation. In spt- embryos, par1 expression is both delayed and severely reduced whereas in flh- embryos, ectopic transcripts are detected in axial mesoderm. Spatial regulation of par1 expression within the somites is affected in several mutants with defects in axial midline tissues.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping