PUBLICATION
Circadian rhythmicity in the locomotor activity of larval zebrafish
- Authors
- Cahill, G.M., Hurd, M.W., and Batchelor, M.M.
- ID
- ZDB-PUB-990105-5
- Date
- 1998
- Source
- Neuroreport 9: 3445-3449 (Journal)
- Registered Authors
- Cahill, Greg, Hurd, Mark W.
- Keywords
- activity cycles; circadian rhythm; computer-assisted; image processing; motor activity; zebrafish
- MeSH Terms
-
- Animals
- Behavior, Animal/physiology
- Circadian Rhythm/physiology*
- Image Processing, Computer-Assisted
- Larva/physiology
- Motor Neurons/physiology*
- Swimming/physiology*
- Videotape Recording
- Zebrafish
- PubMed
- 9855296 Full text @ Neuroreport
Citation
Cahill, G.M., Hurd, M.W., and Batchelor, M.M. (1998) Circadian rhythmicity in the locomotor activity of larval zebrafish. Neuroreport. 9:3445-3449.
Abstract
The zebrafish (Danio rerio) may be useful for mutational analyses of vertebrate circadian clock mechanisms if efficient assays of circadian rhythmicity are available. Using an automated video image analysis system, we found robust circadian rhythms in the locomotor activity of larval (10- to 15-day-old) zebrafish maintained in constant conditions. Activity was rhythmic in > 95% of the animals tested. The timing of peak activity in constant conditions was determined by the prior light:dark cycle, with highest activity during the subjective day. The mean freerunning period of the activity rhythms was 25.6 h, and the within-experiment standard deviation of freerunning period ranged from 0.5 to 1.0 h. Therefore, it should be possible to detect mutations that lengthen or shorten the freerunning circadian period of zebrafish activity rhythms by 1-2 h.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping