PUBLICATION
Chemotopic, combinatorial, and noncombinatorial odorant representations in the olfactory bulb revealed using a voltage-sensitive axon tracer
- Authors
- Friedrich, R.W. and Korsching, S.I.
- ID
- ZDB-PUB-981221-1
- Date
- 1998
- Source
- The Journal of neuroscience : the official journal of the Society for Neuroscience 18: 9977-9988 (Journal)
- Registered Authors
- Friedrich, Rainer, Korsching, Sigrun
- Keywords
- olfactory coding; optical recording; axon tracing; activity pattern; zebrafish; pheromone; olfactory glomerulus; voltage-sensitive dye
- MeSH Terms
-
- Animals
- Axons/physiology*
- Bile Acids and Salts
- Electric Stimulation
- Fluorescent Dyes
- Image Processing, Computer-Assisted
- Microscopy, Fluorescence/methods
- Nucleotides
- Odorants
- Olfactory Bulb/cytology*
- Olfactory Bulb/physiology*
- Olfactory Receptor Neurons/cytology
- Olfactory Receptor Neurons/physiology
- Olfactory Receptor Neurons/ultrastructure
- Pheromones/physiology
- Pyridinium Compounds
- Zebrafish
- PubMed
- 9822753 Full text @ J. Neurosci.
Citation
Friedrich, R.W. and Korsching, S.I. (1998) Chemotopic, combinatorial, and noncombinatorial odorant representations in the olfactory bulb revealed using a voltage-sensitive axon tracer. The Journal of neuroscience : the official journal of the Society for Neuroscience. 18:9977-9988.
Abstract
Odor information is first represented in the brain by patterns of input activity across the glomeruli of the olfactory bulb (OB). To examine how odorants are represented at this stage of olfactory processing, we labeled anterogradely the axons of olfactory receptor neurons with the voltage-sensitive dye Di8-ANEPPQ in zebrafish. The activity induced by diverse natural odorants in afferent axons and across the array of glomeruli was then recorded optically. The results show that certain subregions of the OB are preferentially activated by defined chemical odorant classes. Within these subregions, "ordinary" odorants (amino acids, bile acids, and nucleotides) induce overlapping activity patterns involving multiple glomeruli, indicating that they are represented by combinatorial activity patterns. In contrast, two putative pheromone components (prostaglandin F2alpha and 17alpha, 20beta-dihydroxy-4-pregnene-3-one-20- sulfate) each induce a single focus of activity, at least one of which comes from a single, highly specific and sensitive glomerulus. These results indicate that the OB is organized into functional subregions processing classes of odorants. Furthermore, they suggest that individual odorants can be represented by "combinatorial" or "noncombinatorial" (focal) activity patterns and that the latter may serve to process odorants triggering distinct responses such as that of pheromones.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping