Induction of alpha1-tubulin gene expression during development and regeneration of the fish central nervous system
- Hieber, V., Dai, X.H., Foreman, M., and Goldman, D.
- Journal of neurobiology 37: 429-440 (Journal)
- Registered Authors
- Dai, Xinhua, Goldman, Dan
- CNS development; tubulin; gene expression; regeneration; zebrafish
- MeSH Terms
- Base Sequence
- Central Nervous System/physiology*
- Gene Expression Regulation, Developmental/physiology*
- Genetic Linkage
- In Situ Hybridization
- Molecular Sequence Data
- Nerve Regeneration*
- Optic Nerve Injuries
- Promoter Regions, Genetic
- Retinal Ganglion Cells/metabolism
- Transcriptional Activation
- 9828048 Full text @ J. Neurobiol.
Hieber, V., Dai, X.H., Foreman, M., and Goldman, D. (1998) Induction of alpha1-tubulin gene expression during development and regeneration of the fish central nervous system. Journal of neurobiology. 37:429-440.
The alpha1- and alpha2-tubulin encoding genes were cloned from a goldfish genomic DNA library. alpha1- and alpha2-tubulin RNA expression was examined in developing and adult retinas. These studies demonstrated increased alpha1-tubulin RNA in presumptive ganglion cells that grow axons early in retinal development and in adult retinal ganglion cells whose optic axons had been damaged. The alpha2-tubulin RNA was undetectable in developing retina and constitutively expressed in adult retinal ganglion cells regardless of optic nerve crush. To determine if these changes in alpha1-tubulin RNA reflected changes in alpha1-tubulin promoter activity, we introduced into zebrafish embryos and adult goldfish retinal explants expression vectors harboring the alpha1-tubulin gene's promoter. These studies showed that the alpha1-tubulin promoter confers a developmentally regulated, neuron-restricted pattern of reporter gene expression in vivo and its activity is increased in adult retinal neurons induced to regenerate their axons. Promoter deletions defined regions of alpha1-tubulin DNA necessary for this pattern of expression. These results suggest that DNA sequences necessary for alpha1-tubulin gene induction during central nervous system development and regeneration are contained within the alpha1-tubulin gene's 5'-flanking DNA and that this promoter will be useful for identifying these elements and their DNA binding proteins.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes