PUBLICATION
Characterization of a major permeability barrier in the zebrafish embryo
- Authors
- Hagedorn, M., Kleinhans, F.W., Artemov, D., and Pilatus, U.
- ID
- ZDB-PUB-981105-8
- Date
- 1998
- Source
- Biology of reproduction 59: 1240-1250 (Journal)
- Registered Authors
- Hagedorn, Mary
- Keywords
- none
- MeSH Terms
-
- Animals
- Biophysical Phenomena
- Biophysics
- Blastoderm/metabolism
- Blastoderm/ultrastructure
- Cryopreservation*
- Cryoprotective Agents
- Dimethyl Sulfoxide/metabolism
- Egg Yolk/metabolism
- Egg Yolk/ultrastructure
- Freezing
- Magnetic Resonance Spectroscopy
- Microscopy, Electron
- Permeability
- Water/metabolism
- Zebrafish/embryology*
- PubMed
- 9780333 Full text @ Biol. Reprod.
Citation
Hagedorn, M., Kleinhans, F.W., Artemov, D., and Pilatus, U. (1998) Characterization of a major permeability barrier in the zebrafish embryo. Biology of reproduction. 59:1240-1250.
Abstract
Fish embryos represent a class of multicompartmental biological systems that have not been successfully cryopreserved, primarily because of the lack of understanding of how water and cryoprotectants permeate the compartments. We are using the zebrafish embryo as a model to understand these kinetics. Zebrafish embryos have two major compartments, the blastoderm and the yolk, which is surrounded by the multinucleated yolk syncytial layer (YSL). We determined the water and cryoprotectant permeability in these compartments using two methods. First, we measured shrink/swell dynamics in optical volumetric experiments. Zebrafish embryos shrank over time and did not re-expand while immersed in dimethyl sulfoxide (DMSO) or propylene glycol. Second, we measured DMSO uptake with diffusion-weighted nuclear magnetic resonance spectroscopy. DMSO uptake was rapid during the first few minutes, then gradual thereafter. We used one- and two-compartment models to analyze the data and to determine the permeability parameters. We found that the two-compartment model provided a better fit to the data. On the basis of this model and in the presence of DMSO, the yolk and blastoderm had very similar water permeabilities (i.e., 0.01 and 0. 005 micron x min-1atm-1, respectively), but they had different DMSO permeabilities separated by three orders of magnitude (i.e.,
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping