PUBLICATION
Induction of the zebrafish ventral brain and floorplate requires cyclops/nodal signalling
- Authors
- Sampath, K., Rubinstein, A.L., Cheng, A.H.S., Liang, J.O., Fekany, K., Solnica-Krezel, L., Korzh, V., Halpern, M.E., and Wright, C.V.E.
- ID
- ZDB-PUB-980929-2
- Date
- 1998
- Source
- Nature 395: 185-189 (Journal)
- Registered Authors
- Fekany-Lee, Kimberly, Halpern, Marnie E., Korzh, Vladimir, Liang, Jennifer, Rubinstein, Amy, Sampath, Karuna, Solnica-Krezel, Lilianna, Wright, Christopher V.E.
- Keywords
- none
- MeSH Terms
-
- Embryonic Induction*
- Nodal Protein
- Mutation
- Xenopus Proteins
- Xenopus
- Zebrafish Proteins
- Intracellular Signaling Peptides and Proteins
- Molecular Sequence Data
- Brain/embryology*
- Zebrafish
- Trans-Activators*
- Gastrula/physiology
- Protein Biosynthesis
- Signal Transduction*
- Body Patterning/physiology
- Animals
- Mesoderm/physiology
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/physiology*
- Hedgehog Proteins
- PubMed
- 9744278 Full text @ Nature
Citation
Sampath, K., Rubinstein, A.L., Cheng, A.H.S., Liang, J.O., Fekany, K., Solnica-Krezel, L., Korzh, V., Halpern, M.E., and Wright, C.V.E. (1998) Induction of the zebrafish ventral brain and floorplate requires cyclops/nodal signalling. Nature. 395:185-189.
Abstract
Zebrafish cyclops (cyc) mutations cause deficiencies in the dorsal mesendoderm and ventral neural tube, leading to neural defects and cyclopia. Here we report that cyc encodes a transforming growth factor-beta (TGF-beta)-related intercellular signalling molecule that is similar to mouse nodal. cyc is expressed in dorsal mesendoderm at gastrulation and in the prechordal plate until early somitogenesis. Expression reappears transiently in the left lateral-plate mesoderm, and in an unprecedented asymmetric pattern in the left forebrain. Injection of cyc RNA non-autonomously restores sonic hedgehog-expressing cells of the ventral brain and floorplate that are absent in cyc mutants, whereas inducing activities are abolished by cyc, a mutation of a conserved cysteine in the mature ligand. Our results indicate that cyc provides an essential non-cell-autonomous signal at gastrulation, leading to induction of the floorplate and ventral brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping