PUBLICATION
Comparison of sequence function of the Oct-6 genes in zebrafish, chicken and mouse
- Authors
- Levavasseur, F., Mandemakers, W., Visser, P., Broos, L., Grosveld, F., Zivkovic, D., and Meijer, D.
- ID
- ZDB-PUB-980910-3
- Date
- 1998
- Source
- Mechanisms of Development 74: 89-98 (Journal)
- Registered Authors
- Jongejan-Zivkovic, Dana
- Keywords
- oct-6 gene; Schwann cell differentiation; zebrafish; chicken; mouse; POU genes
- MeSH Terms
-
- Nerve Tissue Proteins/chemistry
- Nerve Tissue Proteins/genetics*
- Nerve Tissue Proteins/physiology
- Animals
- Gene Library
- Zebrafish Proteins*
- Zebrafish/genetics*
- Open Reading Frames
- Animal Structures/embryology
- Animal Structures/innervation
- Myelin Sheath/physiology
- Amino Acid Sequence
- POU Domain Factors
- Octamer Transcription Factor-6
- Species Specificity
- HeLa Cells
- Peripheral Nerves/embryology
- Peripheral Nerves/metabolism
- Transfection
- Chickens/genetics*
- Molecular Sequence Data
- Schwann Cells/physiology
- Structure-Activity Relationship
- Humans
- Gene Expression Regulation
- Protein Structure, Tertiary
- Mice/genetics*
- Transcription Factors/chemistry
- Transcription Factors/genetics*
- Transcription Factors/physiology
- Transcriptional Activation
- Evolution, Molecular
- PubMed
- 9651490 Full text @ Mech. Dev.
Citation
Levavasseur, F., Mandemakers, W., Visser, P., Broos, L., Grosveld, F., Zivkovic, D., and Meijer, D. (1998) Comparison of sequence function of the Oct-6 genes in zebrafish, chicken and mouse. Mechanisms of Development. 74:89-98.
Abstract
To examine the role of the Oct-6 gene in Schwann cell differentiation we have cloned and characterized the chicken and zebrafish homologues of the mouse Oct-6 gene. While highly homologous in the Pit1-Oct1/2-Unc86 (POU) domain, sequence similarities are limited outside this domain. Both genes are intronless and both proteins lack the amino acid repeats that are a characteristic feature of the mammalian Oct-6 proteins. However as in mammals, the aminoterminal parts of the chicken and zebrafish Oct-6 proteins are essential for transactivation of octamer containing promoters. By immunohistochemistry we have found that the chicken Oct-6 protein is expressed in late embryonic ensheathing Schwann cells of the sciatic nerve and is rapidly downregulated when myelination proceeds. This expression profile in glial cells is identical to that in the mouse and rat. Furthermore the zebrafish Oct-6 homolog is expressed in the posterior lateral nerve at a time when it contains actively myelinating Schwann cells. Thus despite extensive primary sequence divergence among the vertebrate Oct-6 proteins, the expression of the chicken and zebrafish Oct-6 proteins is consistent with the notion that Oct-6 functions as a 'competence factor' in promyelin cells to execute the myelination program.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping