ZFIN ID: ZDB-PUB-980812-1
A novel homeobox gene, dharma, can induce the organizer in a non-cell-autonomous manner
Yamanaka, Y., Mizuno, T., Sasai, Y., Kishi, M., Takeda, H., Kim, C.-H., Hibi, M., and Hirano, T.
Date: 1998
Source: Genes & Development   12: 2345-2353 (Journal)
Registered Authors: Hibi, Masahiko, Kim, Cheol-Hee, Mizuno, Toshiro, Takeda, Hiroyuki, Yamanaka, Yojiro
Keywords: organizer; Nieuwkoop center; dharma; zebrafish; yolk syncytial layer
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Body Patterning/genetics*
  • Cloning, Molecular
  • Embryonic Induction/genetics
  • Gene Expression Regulation, Developmental
  • Genes, Homeobox*
  • Homeodomain Proteins/genetics*
  • In Situ Hybridization
  • Intercellular Signaling Peptides and Proteins
  • Molecular Sequence Data
  • Proteins/genetics
  • RNA, Messenger/genetics
  • Xenopus/embryology
  • Xenopus/genetics
  • Xenopus Proteins
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins*
PubMed: 9694799 Full text @ Genes & Dev.
The formation of Spemann organizer is one of the most important steps in dorsoventral axis determination in vertebrate development. However, whether the organizer forms autonomously or is induced non-cell- autonomously is controversial. In this report we have isolated a novel zebrafish homeobox gene, dharma, capable of inducing the organizer ectopically. The expression of dharma was first detected in several blastomeres at one side of the margin soon after the mid-blastula transition and continued in the dorsal side of the yolk syncytial layer (YSL) under the embryonic shield, the zebrafish organizer, until the onset of gastrulation. Furthermore, dharma expressed in the YSL induced the organizer in a non-cell-autonomous manner. These results provided the first identification of a zygotic gene to be implicated in the formation of an organizer-inducing center.